Linked Life Data

16807326

Source:http://linkedlifedata.com/resource/pubmed/id/16807326

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
26
pubmed:dateCreated
2006-6-29
pubmed:abstractText
The cell adhesion molecule N-cadherin has been proposed to regulate synapse formation in mammalian central neurons. This is based on its synaptic localization enabling alignment of presynaptic and postsynaptic specializations by an adhesion mechanism. However, a potential role of N-cadherin in regulating synaptic transmission has remained elusive. In this paper, a functional analysis of N-cadherin knock-out synapses was enabled by in vitro neuronal differentiation of mouse embryonic stem cells circumventing the early embryonic lethality of mice genetically null for N-cadherin. In our in vitro system, initial synapse formation was not altered in the absence of N-cadherin, which might be attributable to compensatory mechanisms. Here, we demonstrate that N-cadherin is required for regulating presynaptic function at glutamatergic synapses. An impairment in the availability of vesicles for exocytosis became apparent selectively during high activity. Short-term plasticity was strongly altered with synaptic depression enhanced in the absence of N-cadherin. Most intriguingly, facilitation was converted to depression under specific stimulation conditions. This indicates an important role of N-cadherin in the control of short-term plasticity. To analyze, whether N-cadherin regulates presynaptic function by a transsynaptic mechanism, we studied chimeric cultures consisting of wild-type neocortical neurons and ES cell-derived neurons. With N-cadherin absent only postsynaptically, we observed a similar increase in short-term synaptic depression as found in its complete absence. This indicates a retrograde control of short-term plasticity by N-cadherin. In summary, our results revealed an unexpected involvement of a synaptic adhesion molecule in the regulation of short-term plasticity at glutamatergic synapses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
28
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6968-78
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:16807326-Animals, pubmed-meshheading:16807326-Cadherins, pubmed-meshheading:16807326-Cell Differentiation, pubmed-meshheading:16807326-Cells, Cultured, pubmed-meshheading:16807326-Electric Conductivity, pubmed-meshheading:16807326-Embryo, Mammalian, pubmed-meshheading:16807326-Exocytosis, pubmed-meshheading:16807326-Fluorescent Dyes, pubmed-meshheading:16807326-Glutamic Acid, pubmed-meshheading:16807326-Mice, pubmed-meshheading:16807326-Mice, Knockout, pubmed-meshheading:16807326-Neuronal Plasticity, pubmed-meshheading:16807326-Neurons, pubmed-meshheading:16807326-Pyridinium Compounds, pubmed-meshheading:16807326-Quaternary Ammonium Compounds, pubmed-meshheading:16807326-Staining and Labeling, pubmed-meshheading:16807326-Stem Cells, pubmed-meshheading:16807326-Sucrose, pubmed-meshheading:16807326-Synapses, pubmed-meshheading:16807326-Synaptic Vesicles, pubmed-meshheading:16807326-Time Factors
pubmed:year
2006
pubmed:articleTitle
N-cadherin transsynaptically regulates short-term plasticity at glutamatergic synapses in embryonic stem cell-derived neurons.
pubmed:affiliation
Lehrstuhl für Zellphysiologie, Ruhr-Universität Bochum, 44780 Bochum, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't