Source:http://linkedlifedata.com/resource/pubmed/id/16807025
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2006-8-22
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| pubmed:abstractText |
A partial gene for eel (Anguilla japonica) vascular endothelial growth factor (VEGF) has been cloned and an endothelial-cell-enriched primary culture derived from rete mirabile established to study regulation of the expression of the eel VEGF gene. Cells were cultured in M199 medium containing 0.1% fetal calf serum (FCS) and serum-free M199 medium for long-and short-term experiments, respectively. Cells were separately treated with cobalt ions (Co2+), basic fibroblast growth factor (bFGF), and estradiol (E2), which have been demonstrated to stimulate mammalian VEGF A expression, followed by quantification of the VEGF mRNA levels by real-time reverse transcription polymerase chain reaction. Our results show that: (1) the deduced eel VEGF protein encoded by the cloned gene is about 130 amino acids in length, and is closely related to a zebrafish (Danio rerio) VEGF A; (2) the endothelial-cell-enriched rete mirabile primary culture containing mainly (over 70%) the capillary endothelial cells; (3) the expression levels of the eel VEGF transcript were increased by Co2+, bFGF, and E2 treatments in a dose-and time-dependent manner. Our data demonstrate that an eel partial VEGF gene has been cloned and its regulation of expression in endothelial-cell-enriched rete mirabile cell culture is similar to that in higher vertebrates.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cobalt,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
1095-6433
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
145
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
33-46
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:16807025-Amino Acid Sequence,
pubmed-meshheading:16807025-Anguilla,
pubmed-meshheading:16807025-Animals,
pubmed-meshheading:16807025-Base Sequence,
pubmed-meshheading:16807025-Cells, Cultured,
pubmed-meshheading:16807025-Cobalt,
pubmed-meshheading:16807025-Culture Media,
pubmed-meshheading:16807025-DNA, Complementary,
pubmed-meshheading:16807025-Dose-Response Relationship, Drug,
pubmed-meshheading:16807025-Endothelial Cells,
pubmed-meshheading:16807025-Estradiol,
pubmed-meshheading:16807025-Fibroblast Growth Factor 2,
pubmed-meshheading:16807025-Gene Expression,
pubmed-meshheading:16807025-Immunohistochemistry,
pubmed-meshheading:16807025-Lipoproteins, LDL,
pubmed-meshheading:16807025-Molecular Sequence Data,
pubmed-meshheading:16807025-Phylogeny,
pubmed-meshheading:16807025-RNA, Messenger,
pubmed-meshheading:16807025-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16807025-Sequence Analysis, DNA,
pubmed-meshheading:16807025-Time Factors,
pubmed-meshheading:16807025-Vascular Endothelial Growth Factor A
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| pubmed:year |
2006
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| pubmed:articleTitle |
An endothelial-cell-enriched primary culture system to study vascular endothelial growth factor (VEGF A) expression in a teleost, the Japanese eel (Anguilla japonica).
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| pubmed:affiliation |
Department of Life Science, National University of Kaohsiung, No. 700, Kaohsiung University Road, Nan Tzu Dist., 811 Kaohsiung, Taiwan. yshuang@nuk.edu.tw
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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