|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2006-11-19
|
|
pubmed:abstractText |
Leukemia-associated antigens such as proteins encoded by MAGE genes might provide tools for immunotherapy of leukemia. Positive and negative results of MAGE-A gene expression in hematological malignancies have been reported. This led us to study MAGE-A gene expression in human leukemias using RT-PCR. Among 115 leukemias from various subtypes, 14/34 (41.17%) AML were positive for one of the three genes analyzed (MAGE-A1 1/32; MAGE-A3 10/32; MAGE-B2 3/12). Expression was also detected in 23/76 (30.26%) B-cell ALL patients (MAGE-A1 2/53; MAGE-A3 20/53; MAGE-B2 1/32). One of these patients expressed both MAGE-A1 (weak signal) and -A3 (strong signal) genes. Other patient with CML were positive for MAGE-B2 (1/5, 20%). MAGE-A3 expression data were corroborated by real time RT-PCR through determination of MAGE-A3 transcript levels. We concluded that the MAGE-A3 gene is expressed at the mRNA level in a proportion of human leukemias.
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0145-2126
|
|
pubmed:author |
pubmed-author:Amancio-ChassinOO,
pubmed-author:CollazoJJ,
pubmed-author:Garrido-GuerreroEE,
pubmed-author:GutiérrezMM,
pubmed-author:MartínezAA,
pubmed-author:MayaniHH,
pubmed-author:McCurdyD KDK,
pubmed-author:MergoldM AMA,
pubmed-author:MirandaE IEI,
pubmed-author:MontesinosJ JJJ,
pubmed-author:OlarteII,
pubmed-author:OrdóñezR MRM,
pubmed-author:Ostrosky-WegmanPP,
pubmed-author:RozenEE
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
31
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
33-7
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:16806467-Adult,
pubmed-meshheading:16806467-Antigens, Neoplasm,
pubmed-meshheading:16806467-Base Sequence,
pubmed-meshheading:16806467-DNA Primers,
pubmed-meshheading:16806467-Female,
pubmed-meshheading:16806467-Gene Amplification,
pubmed-meshheading:16806467-Humans,
pubmed-meshheading:16806467-Leukemia,
pubmed-meshheading:16806467-Leukemia, Myelogenous, Chronic, BCR-ABL Positive,
pubmed-meshheading:16806467-Leukemia, Myeloid, Acute,
pubmed-meshheading:16806467-Male,
pubmed-meshheading:16806467-Middle Aged,
pubmed-meshheading:16806467-Neoplasm Proteins,
pubmed-meshheading:16806467-Precursor Cell Lymphoblastic Leukemia-Lymphoma,
pubmed-meshheading:16806467-RNA, Messenger,
pubmed-meshheading:16806467-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16806467-Transcription, Genetic
|
|
pubmed:year |
2007
|
|
pubmed:articleTitle |
mRNA expression of MAGE-A3 gene in leukemia cells.
|
|
pubmed:affiliation |
Laboratorio de Biología Molecular, Servicio de Hematología, Hospital General de México, Dr. Balmis 148, Col. Doctores, 06726 México City D.F., Mexico.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
