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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-3
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pubmed:dateCreated |
2006-7-24
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pubmed:abstractText |
It has been reported that a prior exposure of isoflurane, a commonly used volatile anesthetic in clinical practice, reduces brain cell death after ischemia. This isoflurane preconditioning-induced neuroprotection has been shown in rat in vivo and in vitro brain ischemia models. To investigate the mechanisms of this protection, we used the human neuroblastoma SH-SY5Y cells and simulated ischemia in vitro by oxygen-glucose deprivation. We found that isoflurane exposure for 30 min at 24 h before a 5-h oxygen-glucose deprivation dose-dependently reduced cell death. Isoflurane exposure induced phosphorylation/activation of extracellular signal-regulated kinase (ERK). Inhibition of the phospho-ERK expression abolished the isoflurane preconditioning-induced protection. Isoflurane exposure also increased the expression of early growth response gene 1 (Egr-1) and Bcl-2, proteins downstream of ERK. Egr-1 is a transcription factor and plays a role in cell survival. Bcl-2 is an anti-apoptotic protein. The increased expression of Egr-1 and Bcl-2 by isoflurane was inhibited by ERK inhibition. Thus, our results suggest a role of ERK/Egr-1/Bcl-2 pathway in the isoflurane preconditioning-induced protection in the human neuroblastoma SH-SY5Y cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Butadienes,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Single-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/DNA enzyme ED5,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Isoflurane,
http://linkedlifedata.com/resource/pubmed/chemical/Neuroprotective Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Nitriles,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/U 0126
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0014-2999
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
542
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
84-91
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:16806162-Butadienes,
pubmed-meshheading:16806162-Cell Hypoxia,
pubmed-meshheading:16806162-Cell Line, Tumor,
pubmed-meshheading:16806162-Cell Survival,
pubmed-meshheading:16806162-DNA, Single-Stranded,
pubmed-meshheading:16806162-Dose-Response Relationship, Drug,
pubmed-meshheading:16806162-Enzyme Inhibitors,
pubmed-meshheading:16806162-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:16806162-Glucose,
pubmed-meshheading:16806162-Humans,
pubmed-meshheading:16806162-Hypoxia-Ischemia, Brain,
pubmed-meshheading:16806162-Isoflurane,
pubmed-meshheading:16806162-Neuroblastoma,
pubmed-meshheading:16806162-Neuroprotective Agents,
pubmed-meshheading:16806162-Nitriles,
pubmed-meshheading:16806162-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:16806162-Reperfusion Injury,
pubmed-meshheading:16806162-Signal Transduction
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pubmed:year |
2006
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pubmed:articleTitle |
Isoflurane preconditioning protects human neuroblastoma SH-SY5Y cells against in vitro simulated ischemia-reperfusion through the activation of extracellular signal-regulated kinases pathway.
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pubmed:affiliation |
Department of Anesthesiology and Neuroscience, University of Virginia, Charlottesville, VA 22908, USA. zz3c@virginia.edu
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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