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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2006-7-28
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pubmed:abstractText |
We established a PC12 cell line (PC12TH Tet-Off) in which human tyrosine hydroxylase (TH) expression can be negatively controlled by Doxycycline (Dox). First, dopamine (DA)-secretion from PC12TH Tet-Off cells was controlled by Dox-administration in a dose-responsive manner ranging from 0 to 100 ng/ml for 70 days in vitro. Furthermore, Parkinson's disease model of rats receiving encapsulated PC12TH Tet-Off cells displayed a significant decrease of dopamine concentration in the cerebrospinal fluid (CSF) and increase of the number of apomorphine-induced rotations by Dox-administration, as compared to transplanted rats without Dox-administration, although the significant decrease of the reduction ratio of DA concentration in the CSF with Dox-administration was recognized over time. At 2 months post-implantation, concentration of dopamine in the implanted striatum and from the retrieved capsules demonstrated that the control of DA-secretion could be partially achieved for 2 months in vivo. Our results support both the value of cell therapy using Tet-Off system and the technique of encapsulation might be a feasible option for Parkinson's disease especially in resolving the problem of dopamine oversupply in the future, although a more efficient way to control DA-secretion with quicker regulation and much titration of dose should be explored before clinical application.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antiporters,
http://linkedlifedata.com/resource/pubmed/chemical/Apomorphine,
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Doxycycline,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidopamine,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine 3-Monooxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/tetA protein, Bacteria
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-8993
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pubmed:author |
pubmed-author:AgariTakashiT,
pubmed-author:DateIsaoI,
pubmed-author:HayaseHitoshiH,
pubmed-author:Ji YuanWenW,
pubmed-author:KamedaMasahiroM,
pubmed-author:KobayashiKazukiK,
pubmed-author:MatsuiToshihiroT,
pubmed-author:MiyoshiYasuyukiY,
pubmed-author:MuraokaKenichiroK,
pubmed-author:ShingoTetsuroT,
pubmed-author:YasuharaTakaoT
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
1102
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-11
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16806124-Animals,
pubmed-meshheading:16806124-Antiporters,
pubmed-meshheading:16806124-Apomorphine,
pubmed-meshheading:16806124-Bacterial Proteins,
pubmed-meshheading:16806124-Behavior, Animal,
pubmed-meshheading:16806124-Cell Transplantation,
pubmed-meshheading:16806124-Disease Models, Animal,
pubmed-meshheading:16806124-Dopamine,
pubmed-meshheading:16806124-Dopamine Agonists,
pubmed-meshheading:16806124-Dose-Response Relationship, Drug,
pubmed-meshheading:16806124-Doxycycline,
pubmed-meshheading:16806124-Female,
pubmed-meshheading:16806124-Gene Expression Regulation,
pubmed-meshheading:16806124-Humans,
pubmed-meshheading:16806124-Immunohistochemistry,
pubmed-meshheading:16806124-Oxidopamine,
pubmed-meshheading:16806124-PC12 Cells,
pubmed-meshheading:16806124-Parkinsonian Disorders,
pubmed-meshheading:16806124-RNA, Messenger,
pubmed-meshheading:16806124-Rats,
pubmed-meshheading:16806124-Rats, Sprague-Dawley,
pubmed-meshheading:16806124-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16806124-Rotarod Performance Test,
pubmed-meshheading:16806124-Time Factors,
pubmed-meshheading:16806124-Tyrosine 3-Monooxygenase
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pubmed:year |
2006
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pubmed:articleTitle |
Control of dopamine-secretion by Tet-Off system in an in vivo model of parkinsonian rat.
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pubmed:affiliation |
Department of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Science, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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