Linked Life Data

16805807

Source:http://linkedlifedata.com/resource/pubmed/id/16805807

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-6-29
pubmed:abstractText
The pain related peptide, calcitonin gene-related peptide (CGRP), plays an important role in inflammatory pain and immune responses. However, its role in neuropathic pain is not established. Following nerve injury, CGRP and pro-inflammatory interleukin-6 (IL-6) are increased in injured nerves. The aim of this study was to determine if CGRP in injured nerves is involved in the up-regulation of IL-6 and in the maintenance of neuropathic pain. Perineural injection of a neutralizing IL-6 antiserum or CGRP receptor antagonists (CGRP8-37 and BIBN4096BS) effectively attenuated thermal hyperalgesia 4 weeks after partial sciatic nerve ligation. Perineural CGRP antagonists also dramatically reduced IL-6 level in injured nerves. CGRP release from injured sites was dramatically increased and CGRP immunoreactivity was localized in both neuroma and invading macrophages. CGRP receptor markers (CRLR and RAMP1) were expressed in invading macrophages. Both CGRP antagonists significantly reduced IL-6 release from injured nerve explants. In cell cultures derived from injured nerves, CGRP concentration-dependently increased IL-6 release, an effect also blocked by CGRP antagonists. Taken together, these data show that increased levels of CGRP in injured neuroma and invading macrophages are involved in the up-regulation of IL-6 in macrophages as well as in the maintenance of neuropathic pain.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
180-92
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16805807-Animals, pubmed-meshheading:16805807-Blotting, Western, pubmed-meshheading:16805807-Calcitonin Gene-Related Peptide, pubmed-meshheading:16805807-Cells, Cultured, pubmed-meshheading:16805807-Disease Models, Animal, pubmed-meshheading:16805807-Dose-Response Relationship, Drug, pubmed-meshheading:16805807-Ectodysplasins, pubmed-meshheading:16805807-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:16805807-Hyperalgesia, pubmed-meshheading:16805807-Immunohistochemistry, pubmed-meshheading:16805807-Interleukin-6, pubmed-meshheading:16805807-Macrophages, pubmed-meshheading:16805807-Male, pubmed-meshheading:16805807-Membrane Proteins, pubmed-meshheading:16805807-Neuroma, pubmed-meshheading:16805807-Pain Measurement, pubmed-meshheading:16805807-Piperazines, pubmed-meshheading:16805807-Quinazolines, pubmed-meshheading:16805807-Rats, pubmed-meshheading:16805807-Rats, Sprague-Dawley, pubmed-meshheading:16805807-Reaction Time, pubmed-meshheading:16805807-Sciatic Neuropathy, pubmed-meshheading:16805807-Tumor Necrosis Factors, pubmed-meshheading:16805807-Up-Regulation
pubmed:year
2006
pubmed:articleTitle
Increased calcitonin gene-related peptide in neuroma and invading macrophages is involved in the up-regulation of interleukin-6 and thermal hyperalgesia in a rat model of mononeuropathy.
pubmed:affiliation
Douglas Hospital Research Center, Department of Psychiatry, McGill University, Montreal, Quebec, Canada.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't