Linked Life Data

1680452

Source:http://linkedlifedata.com/resource/pubmed/id/1680452

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1991-11-21
pubmed:abstractText
Carbohydrates present on the surface of cells have been implicated in such processes as bacterial adherence, surfactant secretion and reutilization, and cell-cell recognition. In this study, fluorescein isothiocyanate (FITC) conjugated lectins were used to probe for such carbohydrates on the surface and interior regions of rabbit peritoneal mesothelial cells propagated in vitro. A cell permeabilization technique employing treatment with formalin and saponin provided the greatest presentation of surface membrane structure and lectin binding. FITC-lectins derived from C. ensiformis (Concanavalin A; mannose specific), T. vulgaris, A. hypogaea, E. cristagalli, B. simplicifolia, and M. pomifera bound to the cell surface. When two strains of Edwarsiella tarda were exposed to the mesothelial cells, only the mannose-specific strain (ET-4) demonstrated substantial adherence to the cell surface.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1197-8554
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
30-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Lectins as probes of membrane carbohydrates in mesothelial cells in vitro.
pubmed:affiliation
University of Illinois College of Medicine, Peoria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't