Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
25
pubmed:dateCreated
2006-6-23
pubmed:abstractText
To test the gliomagenic potential of adult glial progenitors, we infected adult rat white matter with a retrovirus that expresses high levels of PDGF and green fluorescent protein (GFP). Tumors that closely resembled human glioblastomas formed in 100% of the animals by 14 d postinfection. Surprisingly, the tumors were composed of a heterogeneous population of cells, <20% of which expressed the retroviral reporter gene (GFP). The vast majority of both GFP+ and GFP- tumor cells expressed markers of glial progenitors. Thus, the tumors arose from the massive expansion of both infected and uninfected glial progenitors, suggesting that PDGF was driving tumor formation via autocrine and paracrine stimulation of glial progenitor cells. To explore this possibility further, we coinjected a retrovirus expressing PDGF-IRES-DsRed with a control retrovirus expressing only GFP. The resulting tumors contained a mixture of red cells (PDGF-expressing/tumor-initiating cells) and green cells (recruited progenitors). Both populations were highly proliferative and infiltrative. In contrast, when the control GFP retrovirus was injected alone, the animals never formed tumors and the majority of infected cells differentiated along the oligodendrocyte lineage. Together, these results reveal that adult white matter progenitors not only have the capacity to give rise to gliomas, but resident progenitors are recruited to proliferate within the mitogenic environment of the tumor and in this way contribute significantly to the heterogeneous mass of cells that compose a malignant glioma.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1529-2401
pubmed:author
pubmed:issnType
Electronic
pubmed:day
21
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6781-90
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:16793885-Animals, pubmed-meshheading:16793885-Brain Neoplasms, pubmed-meshheading:16793885-Cell Survival, pubmed-meshheading:16793885-Cell Transformation, Neoplastic, pubmed-meshheading:16793885-Cells, Cultured, pubmed-meshheading:16793885-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:16793885-Flow Cytometry, pubmed-meshheading:16793885-Gene Expression Regulation, Neoplastic, pubmed-meshheading:16793885-Glioma, pubmed-meshheading:16793885-Green Fluorescent Proteins, pubmed-meshheading:16793885-Humans, pubmed-meshheading:16793885-Intermediate Filament Proteins, pubmed-meshheading:16793885-Magnetic Resonance Imaging, pubmed-meshheading:16793885-Nerve Tissue Proteins, pubmed-meshheading:16793885-Neuroglia, pubmed-meshheading:16793885-Platelet-Derived Growth Factor, pubmed-meshheading:16793885-Rats, pubmed-meshheading:16793885-Rats, Sprague-Dawley, pubmed-meshheading:16793885-Retroviridae, pubmed-meshheading:16793885-Retroviridae Infections, pubmed-meshheading:16793885-Stem Cells
pubmed:year
2006
pubmed:articleTitle
Glial progenitors in adult white matter are driven to form malignant gliomas by platelet-derived growth factor-expressing retroviruses.
pubmed:affiliation
Department of Pathology, Columbia University, New York, New York 10032, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, N.I.H., Extramural