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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-12-12
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pubmed:abstractText |
To identify transforming genes in acute myeloid leukemia (AML) we here constructed a retroviral cDNA expression library from an AML patient, and then used this library to infect a mouse cell line 32Dcl3-mCAT. cDNA inserts of the cell clones which proliferated in the presence of granulocyte colony-stimulating factor were derived from JAK3 encoding a JAK3 mutant with a valine-to-alanine substitution at codon 674 and two additional amino acid substitutions. The transforming activity of JAK3(V674A) was confirmed by its introduction into 32Dcl3-mCAT. Sequencing of the original JAK3 cDNA derived from the patient, however, failed to detect the V674A mutation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0145-2126
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pubmed:author |
pubmed-author:ChoiYoung LimYL,
pubmed-author:EnomotoMunehiroM,
pubmed-author:FujiwaraShin-IchiroS,
pubmed-author:HatanakaHisashiH,
pubmed-author:KanedaRuriR,
pubmed-author:KurashinaKentaroK,
pubmed-author:ManoHiroyukiH,
pubmed-author:SodaManabuM,
pubmed-author:TakadaShujiS,
pubmed-author:WadaTomoakiT,
pubmed-author:WatanabeHidekiH,
pubmed-author:YamashitaYoshihiroY
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pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
203-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:16790275-Acute Disease,
pubmed-meshheading:16790275-Amino Acid Substitution,
pubmed-meshheading:16790275-Animals,
pubmed-meshheading:16790275-Cell Line,
pubmed-meshheading:16790275-Cell Transformation, Viral,
pubmed-meshheading:16790275-Cloning, Molecular,
pubmed-meshheading:16790275-Gene Expression,
pubmed-meshheading:16790275-Gene Library,
pubmed-meshheading:16790275-Genetic Testing,
pubmed-meshheading:16790275-Humans,
pubmed-meshheading:16790275-Janus Kinase 3,
pubmed-meshheading:16790275-Leukemia, Myeloid,
pubmed-meshheading:16790275-Mice,
pubmed-meshheading:16790275-Mutation,
pubmed-meshheading:16790275-Retroviridae
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pubmed:year |
2007
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pubmed:articleTitle |
Identification of a constitutively active mutant of JAK3 by retroviral expression screening.
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pubmed:affiliation |
Division of Functional Genomics, Jichi Medical University, 3311-1 Yakushiji, Shimotsukeshi, Tochigi 329-0498, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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