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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2006-6-20
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pubmed:abstractText |
Single nucleotide polymorphism analysis (SNP) has recently been proposed as an alternative technique to comparative genomic hybridization (CGH) for defining loss of heterozygosity and gene copy number changes in a single experimental setup. In order to assess the potential of SNP analysis to complement or, ultimately, substitute CGH results, we applied both techniques to five primary tumor samples and two tumor cell lines. This was complemented by dilution experiments based on normal lymphocyte DNA to decipher the lower detection limit for genetic alterations.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1015-2008
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
18-25
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16785764-Algorithms,
pubmed-meshheading:16785764-Cell Line, Tumor,
pubmed-meshheading:16785764-DNA, Neoplasm,
pubmed-meshheading:16785764-DNA Mutational Analysis,
pubmed-meshheading:16785764-Female,
pubmed-meshheading:16785764-Gene Amplification,
pubmed-meshheading:16785764-Gene Dosage,
pubmed-meshheading:16785764-Genetic Techniques,
pubmed-meshheading:16785764-Humans,
pubmed-meshheading:16785764-In Situ Hybridization, Fluorescence,
pubmed-meshheading:16785764-Neoplasms,
pubmed-meshheading:16785764-Polymorphism, Single Nucleotide,
pubmed-meshheading:16785764-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16785764-Sensitivity and Specificity,
pubmed-meshheading:16785764-Software
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pubmed:year |
2006
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pubmed:articleTitle |
Improvements in the analysis strategy make single nucleotide polymorphism analysis a powerful tool in the detection and characterization of amplified chromosomal regions in human tumors.
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pubmed:affiliation |
Institute of Pathology, University of Münster, Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study
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