Linked Life Data

16780873

Source:http://linkedlifedata.com/resource/pubmed/id/16780873

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2006-7-10
pubmed:abstractText
Nuclear import of proteins is determined by specific signals that allow them to bind to receptors that mediate their energy-dependent transport through the nuclear pore. These signals are termed nuclear localization signals and do not constitute a specific consensus sequence. Among them, the most characterized correspond to monopartite and bipartite nuclear localization signals, which interact with the importin alpha/beta heterodimer. We previously described a cytotoxic variant of human pancreatic-ribonuclease that is actively transported into the nucleus. Here, we show that this protein interacts with importin alpha through different basic residues, including Lys1 and the arginine clusters 31-33 and 89-91. Although these residues are scattered along the sequence, they are close in the three-dimensional structure of the protein and their topological disposition strongly resembles that of a classical bipartite nuclear localization signal.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-2836
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
360
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
548-57
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:16780873-Active Transport, Cell Nucleus, pubmed-meshheading:16780873-Amino Acid Sequence, pubmed-meshheading:16780873-Amino Acids, Basic, pubmed-meshheading:16780873-Cell Death, pubmed-meshheading:16780873-Cell Nucleus, pubmed-meshheading:16780873-HeLa Cells, pubmed-meshheading:16780873-Humans, pubmed-meshheading:16780873-Molecular Sequence Data, pubmed-meshheading:16780873-Mutation, pubmed-meshheading:16780873-Nuclear Localization Signals, pubmed-meshheading:16780873-Protein Binding, pubmed-meshheading:16780873-Protein Folding, pubmed-meshheading:16780873-Protein Structure, Secondary, pubmed-meshheading:16780873-Ribonuclease, Pancreatic, pubmed-meshheading:16780873-Smad4 Protein, pubmed-meshheading:16780873-Tumor Cells, Cultured, pubmed-meshheading:16780873-alpha Karyopherins
pubmed:year
2006
pubmed:articleTitle
A cytotoxic ribonuclease variant with a discontinuous nuclear localization signal constituted by basic residues scattered over three areas of the molecule.
pubmed:affiliation
Laboratori d'Enginyeria de Proteïnes, Departament de Biologia, Facultat de Ciències, Universitat de Girona, Campus de Montilivi, 17071 Girona, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't