Linked Life Data

1677197

Source:http://linkedlifedata.com/resource/pubmed/id/1677197

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1309
pubmed:dateCreated
1991-8-16
pubmed:abstractText
Immature Xenopus oocytes injected with inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) give a complex electrophysiological response comprising an a early depolarizing spike followed by a burst of oscillations. These two components have been interpreted on the basis of an interaction between two internal calcium stores: an Ins(1,4,5) P3-sensitive pool responsible for the early spike which then primes an Ins(1,4,5) P3-insensitive pool to begin to oscillate through a process of calcium-induced calcium release (Berridge, M. J., J. Physiol., Lond. 403, 589-599 (1988)). The role of the latter was investigated in Xenopus oocytes by using the drug caffeine which can trigger calcium-induced calcium release in muscle cells. Caffeine had no effect on the early Ins(1,4,5)P3-induced spike but it suppressed the subsequent oscillations. The spontaneous oscillations observed in some oocytes were also abolished by caffeine. Oscillation amplitude and duration was slightly reduced following incubation of oocytes with adenosine or isobutylmethylxanthine. Because these two agents gave large membrane hyperpolarizations indicative of an increase in cyclic AMP, it can be concluded that this second messenger is not responsible for the inhibitory action of caffeine. The ability of caffeine to abolish oscillations while not affecting the early Ins(1,4,5) P3 response is discussed with regard to the two-pool model for generating calcium oscillations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0962-8452
pubmed:author
pubmed:issnType
Print
pubmed:day
22
pubmed:volume
244
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
57-62
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Caffeine inhibits inositol-trisphosphate-induced membrane potential oscillations in Xenopus oocytes.
pubmed:affiliation
University of Cambridge, Department of Zoology, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't