Linked Life Data

16771641

Source:http://linkedlifedata.com/resource/pubmed/id/16771641

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2006-6-14
pubmed:abstractText
The effects of mechanical stimuli on TMJ disc cells have yet to be investigated. This study examined for the first time the effect of constant and intermittent hydrostatic pressure (HP) on TMJ disc cells. Guided by studies on articular chondrocytes, the chosen amplitude was 10 MPa, the frequency was 1 Hz for intermittent HP, and the duration was 4 h. A one-time application of the HP stimulus was applied in 2-D and 3-D for gene expression studies. A duty cycle of 2 days on, 1 day off for 1 week of HP stimulus was used for biochemical content studies. In monolayer, the intermittent HP regimen increased collagen II expression, while constant HP increased collagen I expression when compared to the non-loaded control. However, the overall expression of collagen I was much higher than collagen II in both constant and intermittent HP. The expression results correlated well with gross morphology, histology, and biochemical content. At Week 1, the intermittent HP group had a lower content of collagen, 7.5 +/- 0.2 microg/construct, than the non-loaded control group, 18.2 +/- 4.0 microg/construct. The constant HP group showed the highest amount of collagen, 24.5 +/- 1.6 microg/construct. These data show that constant HP at 10 MPa for 4 h produces more collagen I than do the non-loaded control or intermittent HP at 10 MPa and 1 Hz.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1076-3279
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1285-94
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Effects of hydrostatic pressure on TMJ disc cells.
pubmed:affiliation
Department of Bioengineering, Rice University, Houston, Texas 77251, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural