Source:http://linkedlifedata.com/resource/pubmed/id/16769199
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2006-11-7
|
| pubmed:abstractText |
The surfactant, pulmonary-associated protein C (SFTPC) is a peptide secreted by the alveolar type II pneumocytes of the lung. We have characterized the porcine SFTPC gene at genomic, transcriptional, and protein levels. The porcine SFTPC is a single-copy gene on pig chromosome 14. Two transcripts were found in a newborn pig lung cDNA library: a full-length clone and a clone missing exon 5. cDNA sequence comparison revealed four synonymous and two nonsynonymous substitutions and in-frame insertions at the beginning of exon 5. Comparison of the SFTPC coding region between several mammals showed high levels of conservation. Northern blot studies showed lung-specific expression of the full-length SFTPC transcript, appearing in 50-day-old fetus and increasing during lung development. Both SFTPC transcripts were detected mainly in lung by real-time RT-PCR and they were significantly down-regulated in necrotic lungs of pigs infected with Actinobacillus pleuropneumoniae. Additionally, the protein levels were also decreased or absent in the necrotic tissue.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0888-7543
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
88
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
659-68
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| pubmed:meshHeading |
pubmed-meshheading:16769199-Actinobacillus Infections,
pubmed-meshheading:16769199-Actinobacillus pleuropneumoniae,
pubmed-meshheading:16769199-Amino Acid Sequence,
pubmed-meshheading:16769199-Animals,
pubmed-meshheading:16769199-Animals, Newborn,
pubmed-meshheading:16769199-Base Sequence,
pubmed-meshheading:16769199-DNA, Complementary,
pubmed-meshheading:16769199-Exons,
pubmed-meshheading:16769199-Female,
pubmed-meshheading:16769199-Fetus,
pubmed-meshheading:16769199-Gene Expression Regulation, Developmental,
pubmed-meshheading:16769199-Genomics,
pubmed-meshheading:16769199-Lung,
pubmed-meshheading:16769199-Male,
pubmed-meshheading:16769199-Molecular Sequence Data,
pubmed-meshheading:16769199-Pulmonary Surfactant-Associated Protein C,
pubmed-meshheading:16769199-Sequence Homology, Amino Acid,
pubmed-meshheading:16769199-Sequence Homology, Nucleic Acid,
pubmed-meshheading:16769199-Sus scrofa
|
| pubmed:year |
2006
|
| pubmed:articleTitle |
Molecular characterization of the porcine surfactant, pulmonary-associated protein C gene.
|
| pubmed:affiliation |
Department of Animal and Veterinary Basic Sciences, Division of Genetics, The Royal Veterinary and Agricultural University, 1870 Frederiksberg C, Denmark. scs@kvl.dk
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|