Linked Life Data

16765369

Source:http://linkedlifedata.com/resource/pubmed/id/16765369

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2006-8-14
pubmed:abstractText
An electrokinetically-driven microfluidic chip was developed to realize beads-based solid-phase extraction (SPE) of amino acids. This chip uses a two-level (deep/shallow) poly(dimethylsiloxane) (PDMS) microchannel network to confine the fluorous reversed-phase silica beads within the SPE chamber. The mixture of fluorous tagged and non-tagged amino acids was carried into the fluorous solid-phase extraction (F-SPE) chamber by electrokinetic pumping and was successfully separated and extracted. By adding a reference material to the sample, the extraction efficiency of the eluted fluorous-tagged amino acid was calculated using the detection results from mass spectrometry (MS). The F-SPE microchips showed good reproducibility and efficiency, yielding an average extraction efficiency of 55% with a RSD of 10.6% under the typical experimental conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9797
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
301
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
697-702
pubmed:dateRevised
2009-11-11
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
A microfluidic fluorous solid-phase extraction chip for purification of amino acids.
pubmed:affiliation
Department of Mechanical Engineering, Vanderbilt University, VU Station B 351592, 2301 Vanderbilt Place, Nashville, TN 37235-1592, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't