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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2006-6-9
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pubmed:databankReference |
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pubmed:abstractText |
We report genome-wide analyses that establish Maf1 as a general and direct repressor of yeast RNA polymerase (Pol) III transcription. Chromatin immunoprecipitation (ChIP) coupled to microarray hybridization experiments showed an increased association of Maf1 to Pol III-transcribed genes under repressing condition (rapamycin treatment) correlated with a dissociation of Brf1 and Pol III. Maf1 can exist in various phosphorylation states and interacts with Pol III in a dephosphorylated state. The largest subunit of Pol III, C160, was identified as a target of Maf1. Under repressing conditions, Maf1 is dephosphorylated and accumulates in the nucleus, and Pol III-Maf1 interaction increases. Mutations in protein phosphatase type 2A (PP2A) catalytic subunit-encoding genes prevented rapamycin-induced Maf1 dephosphorylation, its nuclear accumulation, and repression of Pol III transcription. The results indicate that Pol III transcription can be globally and rapidly downregulated via dephosphorylation and relocation of a general negative cofactor.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1097-2765
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
623-32
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16762835-Cell Nucleus,
pubmed-meshheading:16762835-Down-Regulation,
pubmed-meshheading:16762835-Enzyme Repression,
pubmed-meshheading:16762835-Gene Expression Regulation, Fungal,
pubmed-meshheading:16762835-Nuclear Localization Signals,
pubmed-meshheading:16762835-Phosphoprotein Phosphatases,
pubmed-meshheading:16762835-Phosphorylation,
pubmed-meshheading:16762835-Protein Subunits,
pubmed-meshheading:16762835-RNA Polymerase III,
pubmed-meshheading:16762835-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:16762835-Signal Transduction,
pubmed-meshheading:16762835-Sirolimus,
pubmed-meshheading:16762835-Transcription, Genetic,
pubmed-meshheading:16762835-Transcription Factor TFIIIB,
pubmed-meshheading:16762835-Transcription Factors
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pubmed:year |
2006
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pubmed:articleTitle |
General repression of RNA polymerase III transcription is triggered by protein phosphatase type 2A-mediated dephosphorylation of Maf1.
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pubmed:affiliation |
Laboratoire de Transcription des Gènes, Service de Biochimie et Génétique Moléculaire, CEA/Saclay, 91191 Gif-sur-Yvette Cedex, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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