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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 7
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pubmed:dateCreated |
2006-6-8
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pubmed:abstractText |
The proteolytic processing of human immunodeficiency virus (HIV) particles mediated by the viral pol-encoded protease (PR) is essential for viral infectivity. The pol coding sequence partially overlaps with the gag coding sequence and is translated as a Gag-Pol polyprotein precursor. Within Gag-Pol, the C-terminal p6(gag) domain is replaced by a transframe peptide referred to as p6*, which separates the Gag nucleocapsid domain from PR. Several previous in vitro studies have ascribed a PR-suppression regulatory function to p6*. Here, it was demonstrated that an HIV-1 Gag-Pol lacking p6* is efficiently incorporated into virions when coexpressed with HIV-1 Gag precursor. However, the released virions are not processed appropriately and show a greatly reduced viral infectivity. This suggests that the p6* is indispensable during the process of PR-mediated virus particle maturation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2041-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16760407-Amino Acid Sequence,
pubmed-meshheading:16760407-Cell Line,
pubmed-meshheading:16760407-Fusion Proteins, gag-pol,
pubmed-meshheading:16760407-Gene Products, gag,
pubmed-meshheading:16760407-Genes, gag,
pubmed-meshheading:16760407-Genes, pol,
pubmed-meshheading:16760407-HIV Protease,
pubmed-meshheading:16760407-HIV-1,
pubmed-meshheading:16760407-Humans,
pubmed-meshheading:16760407-Molecular Sequence Data,
pubmed-meshheading:16760407-Mutation,
pubmed-meshheading:16760407-Protein Processing, Post-Translational,
pubmed-meshheading:16760407-Virus Assembly,
pubmed-meshheading:16760407-gag Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2006
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pubmed:articleTitle |
Effects of human immunodeficiency virus type 1 transframe protein p6* mutations on viral protease-mediated Gag processing.
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pubmed:affiliation |
Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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