Linked Life Data

16759691

Source:http://linkedlifedata.com/resource/pubmed/id/16759691

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
28
pubmed:dateCreated
2006-6-19
pubmed:abstractText
Efficient cell attachment to biodegradable polymer scaffolds is a necessary prerequisite in tissue engineering. However, it is difficult to evenly cover scaffold surfaces with cells because scaffolds are generally highly porous, with complex three-dimensional (3D) surfaces. In this article, we demonstrate the efficiency of avidin-biotin binding systems (ABBS) for the initial attachment of biotinylated Hep G2 cells to avidin adsorbed flat, two-dimensional (2D) and highly porous 3D poly L-lactic acid (PLLA) surfaces. The potential toxicity of biotinylation and/or strong ABBS binding forces was also investigated. ABBS assisted Hep G2 cells to adhere to a flat PLLA surface within 10min; the proliferation of these attached cells was comparable with control intact cells cultured on collagen. Hepatic functions of the attached cells, such as albumin secretion, induction of CYP1A1 and CYP1A2 genes, and metabolic capacity of CYP1A1/2 as measured by the ethoxyresorufin O-deethylase assay, were not significantly changed. Also, a stimulus of a cytokine: oncostatin M (OSM) phosphorylated an intracellular signaling molecule, extracellular signal-related kinase 1 (ERK1) via transmembrane receptor complex, at 24h after inoculation by ABBS. In addition, efficient attachment of Hep G2 cells to a highly porous PLLA 3D scaffold was demonstrated. These results clearly show that ABBS is useful for rapidly trapping cells in both biodegradable, polymer-based, flat 2D surfaces, and in highly porous 3D scaffolds. Furthermore, binding hepatic cells by this technique has only small effects on liver-specific functions, or on signal transfer ability of transmembrane receptor complexes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0142-9612
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4904-10
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:16759691-Albumins, pubmed-meshheading:16759691-Avidin, pubmed-meshheading:16759691-Biocompatible Materials, pubmed-meshheading:16759691-Biotin, pubmed-meshheading:16759691-Blotting, Western, pubmed-meshheading:16759691-Carcinoma, Hepatocellular, pubmed-meshheading:16759691-Cell Adhesion, pubmed-meshheading:16759691-Cell Proliferation, pubmed-meshheading:16759691-Cytochrome P-450 CYP1A1, pubmed-meshheading:16759691-Cytochrome P-450 CYP1A2, pubmed-meshheading:16759691-Humans, pubmed-meshheading:16759691-Lactic Acid, pubmed-meshheading:16759691-Microscopy, Phase-Contrast, pubmed-meshheading:16759691-Mitogen-Activated Protein Kinase 3, pubmed-meshheading:16759691-Models, Chemical, pubmed-meshheading:16759691-Polymers, pubmed-meshheading:16759691-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16759691-Tissue Engineering, pubmed-meshheading:16759691-Tumor Cells, Cultured
pubmed:year
2006
pubmed:articleTitle
Rapid hepatic cell attachment onto biodegradable polymer surfaces without toxicity using an avidin-biotin binding system.
pubmed:affiliation
Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153 8505 Japan. kojima@iis.u-tokyo.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't