Linked Life Data

16755048

Source:http://linkedlifedata.com/resource/pubmed/id/16755048

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2006-6-6
pubmed:abstractText
Three spectrophotometric methods including Vierordt's method, derivative, ratio spectra derivative, and thin layer chromatography (TLC)-UV densitometric method were developed for simultaneous determination of drotaverine HCl (DRT) and nifuroxazide (NIF) in presence of its impurity, 4-hydroxybenzohydrazide (4-HBH). In Vierordt's method, (E(1 cm)(1%)) values were calculated at 227 and 368 nm in the zero-order spectra of DRT and NIF. By derivative spectrophotometry, the zero-crossing method, drotaverine HCl was determined using the second derivative at 245 nm and the third derivative at 238 nm, while nifuroxazide was determined using the first derivative at 399 nm and the second derivative at 411 nm. The ratio spectra derivative spectrophotometry is based on the measure of the amplitude at 459 nm for DRT and at 416 nm for NIF in the first derivative of the ratio spectra. Calibration graphs of the three spectrophotometric methods were plotted in the range 1-10 microg/ml of DRT and 2-20 microg/ml of NIF. TLC-UV densitometric method was achieved on silica gel plates using ethyl acetate : methanol : ammonia 33% (10 : 1 : 0.1 v/v/v) as the mobile phase. The Rf values were 0.74, 0.50, 0.30+/-0.01 for DRT, NIF and 4-HBH, respectively. On the fluorescent plates, the spots were located by fluorescence quenching and the densitometrical area were measured at 308 and 287 nm with linear range 0.2-4 microg/spot and 0.6-12 microg/spot for DRT and NIF, respectively. The proposed methods have been successfully applied to the commercial pharmaceutical formulation without any interference of excipients. Mean recoveries, relative standard deviations and the results of the proposed methods were compared with those obtained by applying the alternate methods.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1347-5223
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
807-13
pubmed:meshHeading
pubmed-meshheading:16755048-Anti-Infective Agents, pubmed-meshheading:16755048-Calibration, pubmed-meshheading:16755048-Capsules, pubmed-meshheading:16755048-Chemistry, Pharmaceutical, pubmed-meshheading:16755048-Chromatography, High Pressure Liquid, pubmed-meshheading:16755048-Chromatography, Thin Layer, pubmed-meshheading:16755048-Densitometry, pubmed-meshheading:16755048-Drug Stability, pubmed-meshheading:16755048-Hydroxybenzoic Acids, pubmed-meshheading:16755048-Methanol, pubmed-meshheading:16755048-Nitrofurans, pubmed-meshheading:16755048-Papaverine, pubmed-meshheading:16755048-Pharmaceutical Preparations, pubmed-meshheading:16755048-Reference Standards, pubmed-meshheading:16755048-Spectrophotometry, pubmed-meshheading:16755048-Spectrophotometry, Ultraviolet, pubmed-meshheading:16755048-Spectrum Analysis
pubmed:year
2006
pubmed:articleTitle
A comparative study on various spectrometries with thin layer chromatography for simultaneous analysis of drotaverine and nifuroxazide in capsules.
pubmed:affiliation
Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.
pubmed:publicationType
Journal Article