|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
Pt 6
|
|
pubmed:dateCreated |
2006-6-6
|
|
pubmed:abstractText |
L-Rhamnose isomerase from Pseudomonas stutzeri (P. stutzeri L-RhI) catalyzes not only the reversible isomerization of L-rhamnose to L-rhamnulose, but also isomerization between various rare aldoses and ketoses. Purified His-tagged P. stutzeri L-RhI was crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 74.3, b = 104.0, c = 107.0 A, beta = 106.8 degrees . Diffraction data have been collected to 2.0 A resolution. The molecular weight of the purified P. stutzeri L-RhI with a His tag at the C-terminus was confirmed to be 47.7 kDa by MALDI-TOF mass-spectrometric analysis and the asymmetric unit is expected to contain four molecules.
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
1744-3091
|
|
pubmed:author |
|
|
pubmed:issnType |
Electronic
|
|
pubmed:day |
1
|
|
pubmed:volume |
62
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
550-2
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:16754978-Aldose-Ketose Isomerases,
pubmed-meshheading:16754978-Carbohydrate Epimerases,
pubmed-meshheading:16754978-Crystallization,
pubmed-meshheading:16754978-Histidine,
pubmed-meshheading:16754978-Molecular Weight,
pubmed-meshheading:16754978-Pseudomonas stutzeri,
pubmed-meshheading:16754978-Rhamnose,
pubmed-meshheading:16754978-Solvents,
pubmed-meshheading:16754978-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:16754978-X-Ray Diffraction
|
|
pubmed:year |
2006
|
|
pubmed:articleTitle |
Crystallization and preliminary X-ray diffraction studies of L-rhamnose isomerase from Pseudomonas stutzeri.
|
|
pubmed:affiliation |
Molecular Structure Research Group, Information Technology Center and Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho, Kita-gun, Kagawa 761-0793, Japan.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
