Linked Life Data

16753815

Source:http://linkedlifedata.com/resource/pubmed/id/16753815

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2006-6-6
pubmed:databankReference
pubmed:abstractText
A subtracted cDNAs library was constructed using rice (Oryza sativa L.) calli cDNA as driver and differentiating calli cDNA as tester. A novel gene homologous with FCA in Arabidopsis was cloned from rice by screening the SSH (suppression subtractive hybridization) library followed by RACE. Four alternative transcripts of OsFCA were cloned from the leaves of rice, and designated as OsFCA-1, OsFCA-2, OsFCA-3 and OsFCA-4 respectively. OsFCA-1 was homologous to FCA-gamma of Arabidopsis and contained several conserved domains (two RNA Recognition Motifs and one WW-domain). OsFCA-2 was 102 bp shorter than OsFCA-1 which caused the WW-domain deletion. The proteins encoded by OsFCA-3 and OsFCA-4 were 101 amino acids shorter than OsFCA-1 at the N-terminal which is a glycine-rich region. The fluorescence quantitative PCR analysis showed that the mRNA of OsFCA-1 is the most abundant in the four splicing variants of rice FCA, and its expression level is much higher in differentiating calli than in calli. The expression of OsFCA-1 is steady in the leaves of three different stage, but up-regulated in young spikelet of primary branch-differentiating stage and down-regulated in young spikelet of pistil and stamen-differentiating stage.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1042-5179
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
31-40
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Alternative splicing and expression analysis of OsFCA (FCA in Oryza sativa L.), a gene homologous to FCA in Arabidopsis.
pubmed:affiliation
Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, China.
pubmed:publicationType
Journal Article, Comparative Study