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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2006-6-5
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pubmed:abstractText |
Membrane-associated guanylate kinase inverted (MAGI)-1 plays a role as a scaffold at cell junctions in non-neuronal cells, while S-SCAM, its neuronal isoform, is involved in the organization of synapses. A search for MAGI-1-interacting proteins by yeast two-hybrid screening of a kidney cDNA library yielded dendrin. As dendrin was originally reported as a brain-specific postsynaptic protein, we tested the interaction between dendrin and S-SCAM and revealed that dendrin binds to the WW domains of S-SCAM. Dendrin is known to be dendritically translated but its function is largely unknown. To gain insights into the physiological meaning of the interaction, we performed a second yeast two-hybrid screening using dendrin as a bait. We identified CIN85, an endocytic scaffold protein, as a putative dendrin-interactor. Immunocytochemistry and subcellular fractionation analysis supported the synaptic localization of CIN85. The first SH3 domain and the C-terminal region of CIN85 bind to the proline-rich region and the N-terminal region of dendrin, respectively. In vitro experiments suggest that dendrin forms a ternary complex with CIN85 and S-SCAM and that this complex formation facilitates the recruitment of dendrin and S-SCAM to vesicle-like structures where CIN85 is accumulated.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/MAGI-1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Magi2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SH3KBP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/dendrin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-924X
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pubmed:author |
pubmed-author:FujiwaraNaoyukiN,
pubmed-author:HamanoMamikoM,
pubmed-author:HataYutakaY,
pubmed-author:HirabayashiSusumuS,
pubmed-author:IidaJunkoJ,
pubmed-author:IkedaMitsunobuM,
pubmed-author:KansakuAiA,
pubmed-author:KawataAkiraA,
pubmed-author:MoriHirokiH,
pubmed-author:RokukawaChiakiC,
pubmed-author:SatoYujiY,
pubmed-author:SumitaKazutakaK
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pubmed:issnType |
Print
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pubmed:volume |
139
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
931-9
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pubmed:dateRevised |
2011-7-12
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pubmed:meshHeading |
pubmed-meshheading:16751601-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:16751601-Amino Acid Motifs,
pubmed-meshheading:16751601-Animals,
pubmed-meshheading:16751601-Brain,
pubmed-meshheading:16751601-Carrier Proteins,
pubmed-meshheading:16751601-Guanylate Kinase,
pubmed-meshheading:16751601-Humans,
pubmed-meshheading:16751601-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:16751601-Kidney,
pubmed-meshheading:16751601-Membrane Proteins,
pubmed-meshheading:16751601-Nerve Tissue Proteins,
pubmed-meshheading:16751601-Protein Binding,
pubmed-meshheading:16751601-Protein Structure, Tertiary,
pubmed-meshheading:16751601-Rats,
pubmed-meshheading:16751601-Signal Transduction,
pubmed-meshheading:16751601-Synapses,
pubmed-meshheading:16751601-Tissue Distribution,
pubmed-meshheading:16751601-Two-Hybrid System Techniques
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pubmed:year |
2006
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pubmed:articleTitle |
CIN85 is localized at synapses and forms a complex with S-SCAM via dendrin.
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pubmed:affiliation |
Department of Medical Biochemistry, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113-8519.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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