| pubmed-article:167479 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:167479 | lifeskim:mentions | umls-concept:C0034804 | lld:lifeskim |
| pubmed-article:167479 | lifeskim:mentions | umls-concept:C0023688 | lld:lifeskim |
| pubmed-article:167479 | lifeskim:mentions | umls-concept:C0678640 | lld:lifeskim |
| pubmed-article:167479 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:167479 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:167479 | pubmed:dateCreated | 1975-10-10 | lld:pubmed |
| pubmed-article:167479 | pubmed:abstractText | Cytoplasmic estrogen receptor can exist either free (R) or bound to estradiol-17beta (RE). Both forms can be precipitated from cytosols by protamine sulfate. After protamine precipitation R binds 3-H-estradiol-17beta quantitatively at either 0 degrees or 30 degrees, while precipitated RE binds 3-H-estradiol-17beta only at 30 degrees by exchanging with previously bound hormone. Using these observations, we have developed a method for separate determination of both cytoplasmic R and RE. This method should also be applicable for assay of other steroid receptors, especially in cases where interfering components are present in the whole cytosol. | lld:pubmed |
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| pubmed-article:167479 | pubmed:language | eng | lld:pubmed |
| pubmed-article:167479 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:167479 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:167479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:167479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:167479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:167479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:167479 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:167479 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:167479 | pubmed:month | May | lld:pubmed |
| pubmed-article:167479 | pubmed:issn | 0039-128X | lld:pubmed |
| pubmed-article:167479 | pubmed:author | pubmed-author:McGuireW LWL | lld:pubmed |
| pubmed-article:167479 | pubmed:author | pubmed-author:ChamnessG CGC | lld:pubmed |
| pubmed-article:167479 | pubmed:author | pubmed-author:HuffKK | lld:pubmed |
| pubmed-article:167479 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:167479 | pubmed:volume | 25 | lld:pubmed |
| pubmed-article:167479 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:167479 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:167479 | pubmed:pagination | 627-35 | lld:pubmed |
| pubmed-article:167479 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:167479 | pubmed:otherAbstract | PIP: A method for separate determination of cytoplasmic free and bound es trogen receptors to estradiol-17beta using protamine precipitation and ligand exchagne is described. Following protamine precipitation the free binds tritiated-estradiol-17beta quantitatively at 0 or 30 degrees while precipitated bound receptor binds only at 30 degrees by exchanging with previously bound hormone. This method may also be useful for assay of other steroid receptors. | lld:pubmed |
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| pubmed-article:167479 | pubmed:year | 1975 | lld:pubmed |
| pubmed-article:167479 | pubmed:articleTitle | Protamine-precipitated estrogen receptor: a solid-phase ligand exchange assay. | lld:pubmed |
| pubmed-article:167479 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:167479 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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