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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1975-10-10
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pubmed:abstractText |
Cytoplasmic estrogen receptor can exist either free (R) or bound to estradiol-17beta (RE). Both forms can be precipitated from cytosols by protamine sulfate. After protamine precipitation R binds 3-H-estradiol-17beta quantitatively at either 0 degrees or 30 degrees, while precipitated RE binds 3-H-estradiol-17beta only at 30 degrees by exchanging with previously bound hormone. Using these observations, we have developed a method for separate determination of both cytoplasmic R and RE. This method should also be applicable for assay of other steroid receptors, especially in cases where interfering components are present in the whole cytosol.
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pubmed:keyword |
http://linkedlifedata.com/resource/pubmed/keyword/Biology,
http://linkedlifedata.com/resource/pubmed/keyword/Endocrine System,
http://linkedlifedata.com/resource/pubmed/keyword/Estradiol--analysis,
http://linkedlifedata.com/resource/pubmed/keyword/Estrogens,
http://linkedlifedata.com/resource/pubmed/keyword/Examinations And Diagnoses,
http://linkedlifedata.com/resource/pubmed/keyword/Hormone Receptors,
http://linkedlifedata.com/resource/pubmed/keyword/Hormones,
http://linkedlifedata.com/resource/pubmed/keyword/Laboratory Examinations And Diagnoses,
http://linkedlifedata.com/resource/pubmed/keyword/Laboratory Procedures,
http://linkedlifedata.com/resource/pubmed/keyword/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/keyword/Physiology
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0039-128X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
627-35
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pubmed:dateRevised |
2009-11-19
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pubmed:otherAbstract |
PIP: A method for separate determination of cytoplasmic free and bound es trogen receptors to estradiol-17beta using protamine precipitation and ligand exchagne is described. Following protamine precipitation the free binds tritiated-estradiol-17beta quantitatively at 0 or 30 degrees while precipitated bound receptor binds only at 30 degrees by exchanging with previously bound hormone. This method may also be useful for assay of other steroid receptors.
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pubmed:meshHeading |
pubmed-meshheading:167479-Animals,
pubmed-meshheading:167479-Castration,
pubmed-meshheading:167479-Charcoal,
pubmed-meshheading:167479-Chemical Precipitation,
pubmed-meshheading:167479-Cytoplasm,
pubmed-meshheading:167479-Dextrans,
pubmed-meshheading:167479-Estradiol,
pubmed-meshheading:167479-Female,
pubmed-meshheading:167479-Kinetics,
pubmed-meshheading:167479-Ligands,
pubmed-meshheading:167479-Methods,
pubmed-meshheading:167479-Ovary,
pubmed-meshheading:167479-Protamines,
pubmed-meshheading:167479-Rats,
pubmed-meshheading:167479-Receptors, Cell Surface,
pubmed-meshheading:167479-Temperature,
pubmed-meshheading:167479-Tritium,
pubmed-meshheading:167479-Uterus
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pubmed:year |
1975
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pubmed:articleTitle |
Protamine-precipitated estrogen receptor: a solid-phase ligand exchange assay.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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