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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2006-6-2
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pubmed:abstractText |
The 5'-untranslated region (5'UTR) of the fliC flagellin gene of Salmonella contains sequences critical for efficient fliC mRNA translation coupled to assembly. In a previous study we used targeted mutagenesis of the 5' end of the fliC gene to isolate single base changes defective in fliC gene translation. This identified a predicted stem-loop structure, SL2, as an effector of normal fliC mRNA translation. A single base change (-38C:U) in the fliC 5'UTR resulted in a mutant that is defective in fliC mRNA translation and was chosen for this study. Motile (Mot+) revertants of the -38C:T mutant were isolated and characterized, yielding several unexpected results. Second-site suppressors that restored fliC translation and motility included mutations that disrupt a RNA duplex stem formed between RNA sequences in the fliC 5'UTR SL2 region (including a precise deletion of SL2) and bases early within the fliC-coding region. A stop codon mutation at position 80 of flgM also suppressed the -38C:T motility defect, while flgM mutants defective in anti-sigma28 activity had no effect on fliC translation. One remarkable mutation in the fliC 5'UTR (-15G:A) results in a translation defect by itself but, in combination with the -38C:U mutation, restores normal translation. These results suggests signals intrinsic to the fliC mRNA that have both positive and negative effects on fliC translation involving both RNA structure and interacting proteins.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-10096082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-10329189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-10586520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-10829079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-10966110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-10972838,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-11029689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-11104815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-12775694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-12904785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-13278318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-15546667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-15766542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-16153172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-16561620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-16740955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-1943777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-2196428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-2404955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-4564719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-7768847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-7961507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-8028576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-8235660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-8748030,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/16740956-9180698
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
188
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4497-507
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:16740956-5' Untranslated Regions,
pubmed-meshheading:16740956-Bacterial Proteins,
pubmed-meshheading:16740956-Base Sequence,
pubmed-meshheading:16740956-Flagella,
pubmed-meshheading:16740956-Flagellin,
pubmed-meshheading:16740956-Gene Expression Regulation,
pubmed-meshheading:16740956-Locomotion,
pubmed-meshheading:16740956-Molecular Sequence Data,
pubmed-meshheading:16740956-Mutation,
pubmed-meshheading:16740956-Nucleic Acid Conformation,
pubmed-meshheading:16740956-Open Reading Frames,
pubmed-meshheading:16740956-Protein Biosynthesis,
pubmed-meshheading:16740956-RNA, Bacterial,
pubmed-meshheading:16740956-Repressor Proteins,
pubmed-meshheading:16740956-Salmonella typhimurium
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pubmed:year |
2006
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pubmed:articleTitle |
Translation inhibition of the Salmonella fliC gene by the fliC 5' untranslated region, fliC coding sequences, and FlgM.
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pubmed:affiliation |
Department of Microbiology, University of Utah, Salt Lake City, UT 84112, USA.
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pubmed:publicationType |
Journal Article,
Comment,
Research Support, N.I.H., Extramural
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