Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1991-6-13
|
| pubmed:abstractText |
We obtained peripheral blood mononuclear cells (PBMC) from four healthy, tuberculin purified protein derivative (PPD) reactive donors and cultured these cells in media containing PPD (low dose = 200 ng/ml or high dose = 1 micrograms/ml). Five days after the addition of PPD, T cells were isolated, washed, and added to autologous adherent cell cultures at a 1:1 ratio. Adherent cells were then cultured for 24 h in media only (baseline), media plus lipopolysaccharide (LPS, 2 micrograms/ml; positive control), or media containing the prestimulated T cells. After 24 h, supernatants were harvested and interleukin 1 beta (IL-beta) levels were assayed by radioimmunoassay (RIA) or enzyme-linked immunosorbent assay (ELISA). The results show that T cells prestimulated with low dose PPD (200 micrograms/ml) did not induce IL-1 production by adherent cells (mean increase over baseline 0.2 +/- 1.3 standard deviation [SD] ng/ml, P = 0.61). However, T cells prestimulated with high dose PPD (1 microgram/ml) did induce adherent cells to secrete IL-1 beta (mean increase over baseline 1.7 +/- 0.62 [SD] ng/ml, P = 0.01), but this induction was abolished when cell-to-cell contact was prevented by use of double well chambers (mean increase over baseline 0.1 +/- 0.36 [SD] ng/ml, P = 0.69). Prestimulated T helper (CD4+) cells were able to induce monocytes to secrete IL-1 beta but prestimulated CD8+ T cells were not. These data suggest that when T helper (CD4+) cells are sufficiently activated they acquire the ability to induce monocytes to secrete IL-1 beta. Cell-to-cell contact between monocytes and T cells is required. This function of activated T cells may be important in the normal cellular immune response.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0741-5400
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
49
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
542-7
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1673991-CD4-Positive T-Lymphocytes,
pubmed-meshheading:1673991-Cell Communication,
pubmed-meshheading:1673991-Cell Separation,
pubmed-meshheading:1673991-Cells, Cultured,
pubmed-meshheading:1673991-Dose-Response Relationship, Drug,
pubmed-meshheading:1673991-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1673991-Humans,
pubmed-meshheading:1673991-Interleukin-1,
pubmed-meshheading:1673991-Lymphocyte Activation,
pubmed-meshheading:1673991-Monocytes,
pubmed-meshheading:1673991-Radioimmunoassay,
pubmed-meshheading:1673991-T-Lymphocytes, Helper-Inducer,
pubmed-meshheading:1673991-T-Lymphocytes, Regulatory,
pubmed-meshheading:1673991-Tuberculin
|
| pubmed:year |
1991
|
| pubmed:articleTitle |
T helper/inducer (CD4+) cells prestimulated with PPD induce monocytes to produce interleukin-1 beta.
|
| pubmed:affiliation |
Department of Medicine, University of Alabama, Birmingham 35294.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|