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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
3
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pubmed:dateCreated |
1991-6-5
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pubmed:databankReference |
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pubmed:abstractText |
We have isolated a cDNA encoding human epidermal transglutaminase, a key enzyme of terminal differentiation of keratinocytes. A cDNA library from cultured human keratinocytes was screened by a PCR-amplified partial cDNA fragment of the enzyme with oligonucleotide primers based on the homology of the transglutaminase family. The cDNA is 2734 bp coding a protein of 817 amino acids. The several regions including the active site cysteine residue are highly conserved among the transglutaminase family. However, the charged N-terminal domain is unique to the epidermal transglutaminse, suggesting that the region is involved in the function of the enzyme in keratinocytes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
175
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
906-13
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1673840-Amino Acid Sequence,
pubmed-meshheading:1673840-Animals,
pubmed-meshheading:1673840-Base Sequence,
pubmed-meshheading:1673840-Binding Sites,
pubmed-meshheading:1673840-Cells, Cultured,
pubmed-meshheading:1673840-Cloning, Molecular,
pubmed-meshheading:1673840-DNA,
pubmed-meshheading:1673840-Epidermis,
pubmed-meshheading:1673840-Humans,
pubmed-meshheading:1673840-Keratinocytes,
pubmed-meshheading:1673840-Molecular Sequence Data,
pubmed-meshheading:1673840-Oligonucleotide Probes,
pubmed-meshheading:1673840-Polymerase Chain Reaction,
pubmed-meshheading:1673840-Protein Conformation,
pubmed-meshheading:1673840-Restriction Mapping,
pubmed-meshheading:1673840-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1673840-Transglutaminases
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pubmed:year |
1991
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pubmed:articleTitle |
Molecular cloning of human epidermal transglutaminase cDNA from keratinocytes in culture.
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pubmed:affiliation |
Department of Dermatology, Kyoto Prefectural University of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study
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