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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2006-7-28
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pubmed:abstractText |
The main limitation in performing genome-wide gene-expression profiling is the assay of low-expression genes. Approaches with high throughput and high sensitivity for assaying low-expression transcripts are urgently needed for functional genomic studies. Combination of the suppressive subtractive hybridization (SSH) and cDNA microarray techniques using the subtracted cDNA clones as probes printed on chips has greatly improved the efficiency for fishing out the differentially expressed clones and has been used before. However, it remains tedious and inefficient sequencing works for identifying genes including the great number of redundancy in the subtracted amplicons, and sacrifices the original advantages of high sensitivity of SSH in profiling low-expression transcriptomes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-10037815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-10349654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-12100584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-12215257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-12610505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-14715686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-14871811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-14970460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-15176476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-15744023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-15774023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-15791650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-15987785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-16030349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-16049915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-16084687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-16174304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-16249312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-7569999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-7623854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-8127676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-8650213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-9419376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-9461476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-9521924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-9611255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16737534-9634850
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2164
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
131
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pubmed:dateRevised |
2010-9-15
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pubmed:meshHeading |
pubmed-meshheading:16737534-Carcinoma, Hepatocellular,
pubmed-meshheading:16737534-Gene Expression Profiling,
pubmed-meshheading:16737534-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:16737534-Genes, Neoplasm,
pubmed-meshheading:16737534-Humans,
pubmed-meshheading:16737534-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:16737534-Transcription, Genetic
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pubmed:year |
2006
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pubmed:articleTitle |
Differentially profiling the low-expression transcriptomes of human hepatoma using a novel SSH/microarray approach.
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pubmed:affiliation |
Liver Research Unit, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan. extraganoderma@yahoo.com.tw
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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