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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2006-6-12
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pubmed:abstractText |
Histone acetyltransferase 1 (HAT1) is implicated for diacetylation of Lys-5 and Lys-12 of newly synthesized histone H4, the biological significance of which remains unclear. To investigate the in vivo role of HAT1, we generated HAT1-deficient DT40 clone (HAT1(-/-)). HAT1(-/-) cells exhibited greatly reduced diacetylation levels of Lys-5 and Lys-12, and acetylation level of Lys-5 of cytosolic and chromatin histones H4, respectively. The in vitro nucleosome assembly assay and in vivo MNase digestion assay revealed that HAT1 and diacetylation of Lys-5 and Lys-12 of histone H4 are dispensable for replication-coupled chromatin assembly. HAT1(-/-) cells had mild growth defect, conferring sensitivities to methyl methanesulfonate and camptothecin that enforce replication blocks creating DNA double strand breaks. Such heightened sensitivities were associated with prolonged late-S/G2 phase. These results indicate that HAT1 participates in recovering replication block-mediated DNA damages, probably through chromatin modulation based on acetylation of Lys-5 and Lys-12 of histone H4.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
345
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1547-57
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16735025-Acetylation,
pubmed-meshheading:16735025-Animals,
pubmed-meshheading:16735025-Antineoplastic Agents, Alkylating,
pubmed-meshheading:16735025-Blotting, Western,
pubmed-meshheading:16735025-Camptothecin,
pubmed-meshheading:16735025-Cell Line, Tumor,
pubmed-meshheading:16735025-Cell Proliferation,
pubmed-meshheading:16735025-Cell Survival,
pubmed-meshheading:16735025-Chickens,
pubmed-meshheading:16735025-Chromatin,
pubmed-meshheading:16735025-DNA Damage,
pubmed-meshheading:16735025-DNA Repair,
pubmed-meshheading:16735025-DNA Replication,
pubmed-meshheading:16735025-G2 Phase,
pubmed-meshheading:16735025-HeLa Cells,
pubmed-meshheading:16735025-Histone Acetyltransferases,
pubmed-meshheading:16735025-Histones,
pubmed-meshheading:16735025-Humans,
pubmed-meshheading:16735025-Kinetics,
pubmed-meshheading:16735025-Lysine,
pubmed-meshheading:16735025-Methyl Methanesulfonate,
pubmed-meshheading:16735025-Microscopy, Fluorescence,
pubmed-meshheading:16735025-Mutation,
pubmed-meshheading:16735025-S Phase
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pubmed:year |
2006
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pubmed:articleTitle |
Histone acetyltransferase 1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells.
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pubmed:affiliation |
Section of Biochemistry and Molecular Biology, Department of Medical Sciences, Miyazaki Medical College, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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