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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2006-5-31
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pubmed:abstractText |
Bacteria communicate with one another and with their host using chemical signalling molecules. This phenomenon is generally described as quorum sensing. A set of primers for PCR detection of Salmonella spp. has been designed using as target the sdiA gene which encodes a signal receptor of the LuxR family. The PCR product (274 bp) was confirmed by sequencing. A number of 81 non-Salmonella strains (representing 24 different species) were tested and gave negative results, while a total of 101 different serotypes of Salmonella (155 strains) tested positive for the presence of the sdiA gene. The sensitivity and specificity of the sdiA-based PCR assay were also checked in artificially contaminated human faecal samples. In this study, we demonstrate that quorum sensing genes can be successfully exploited as diagnostic markers.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0378-1097
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
259
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
201-7
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pubmed:meshHeading |
pubmed-meshheading:16734780-Animals,
pubmed-meshheading:16734780-Bacterial Proteins,
pubmed-meshheading:16734780-Base Sequence,
pubmed-meshheading:16734780-DNA, Bacterial,
pubmed-meshheading:16734780-DNA Primers,
pubmed-meshheading:16734780-Feces,
pubmed-meshheading:16734780-Genes, Bacterial,
pubmed-meshheading:16734780-Humans,
pubmed-meshheading:16734780-Molecular Sequence Data,
pubmed-meshheading:16734780-Polymerase Chain Reaction,
pubmed-meshheading:16734780-Salmonella enterica,
pubmed-meshheading:16734780-Sensitivity and Specificity,
pubmed-meshheading:16734780-Trans-Activators
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pubmed:year |
2006
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pubmed:articleTitle |
PCR detection of Salmonella spp. using primers targeting the quorum sensing gene sdiA.
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pubmed:affiliation |
Department of Dietetics and Nutritional Science, Harokopio University, Athens, Greece.
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pubmed:publicationType |
Journal Article
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