Source:http://linkedlifedata.com/resource/pubmed/id/16728405
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
31
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pubmed:dateCreated |
2006-7-31
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pubmed:abstractText |
The moss Physcomitrella patens contains high levels of arachidonic acid and lesser amounts of eicosapentaenoic acid. Here we report the identification and characterization of a delta5-desaturase from P. patens that is associated with the synthesis of these fatty acids. A full-length cDNA for this desaturase was identified by data base searches based on homology to sequences of known delta5-desaturase cDNAs from fungal and algal species. The resulting P. patens cDNA encodes a 480-amino acid polypeptide that contains a predicted N-terminal cytochrome b5-like domain as well as three histidine-rich domains. Expression of the enzyme in Saccharomyces cerevisiae resulted in the production of the delta5-containing fatty acid arachidonic acid in cells that were provided di-homo-gamma-linolenic acid. In addition, the expressed enzyme generated delta5-desaturation products with the C20 substrates omega-6 eicosadienoic and omega-3 eicosatrienoic acids, but no products were detected with the C18 fatty acid linoleic and alpha-linolenic acids or with the C22 fatty acid adrenic and docosapentaenoic acids. When the corresponding P. patens genomic sequence was disrupted by replacement through homologous recombination, a dramatic alteration in the fatty acid composition was observed, i.e. an increase in di-homo-gamma-linolenic and eicosatetraenoic acids accompanied by a concomitant disappearance of the delta5-fatty acid arachidonic and eicosapentaenoic acids. In addition, overexpression of the P. patens cDNA in protoplasts isolated from a disrupted line resulted in the restoration of arachidonic acid synthesis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Eicosanoic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Eicosapentaenoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acid Desaturases,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/delta-5 fatty acid desaturase
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
281
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
21988-97
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:16728405-Arachidonic Acid,
pubmed-meshheading:16728405-Bryophyta,
pubmed-meshheading:16728405-Bryopsida,
pubmed-meshheading:16728405-Cloning, Molecular,
pubmed-meshheading:16728405-DNA, Complementary,
pubmed-meshheading:16728405-Eicosanoic Acids,
pubmed-meshheading:16728405-Eicosapentaenoic Acid,
pubmed-meshheading:16728405-Fatty Acid Desaturases,
pubmed-meshheading:16728405-Plant Proteins,
pubmed-meshheading:16728405-Protoplasts
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pubmed:year |
2006
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pubmed:articleTitle |
Identification and functional characterization of the moss Physcomitrella patens delta5-desaturase gene involved in arachidonic and eicosapentaenoic acid biosynthesis.
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pubmed:affiliation |
Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhya Road, Ratchathewi, Bangkok 10400, Thailand.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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