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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2006-6-12
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pubmed:abstractText |
Sarco/endoplasmic reticulum Ca(2+)ATPases (SERCAs) pump free Ca(2+) from the cytosol into the endoplasmic reticulum. The human SERCA3 family counts six members named SERCA3a to 3f. However, the exact role of these different isoforms in cellular physiology remains undetermined. In this study, we compared some physiological consequences of SERCA3b and SERCA3f overexpression in HEK-293 cells. We observed that overexpression of SERCA3b affected cell adhesion capacity associated with a major disorganization of F-actin and a decrease in focal adhesion. Furthermore, we found that SERCA3f overexpression resulted in an increase in endoplasmic reticulum stress markers (including processing of X-box-binding protein-1 (XBP-1) mRNA and expression of chaperone glucose-regulated protein 78 (GRP78)). This was associated with the activation of caspase cascade and a higher spontaneous cell death. In conclusion, these data point for the first time to distinct physiological roles of SERCA3 isoforms in cell functions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP2A3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Transporting ATPases,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Proteinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sarcoplasmic Reticulum...,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/benzoylcarbonyl-aspartyl-glutamyl-va...,
http://linkedlifedata.com/resource/pubmed/chemical/regulatory factor X transcription...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
345
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1377-85
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:16725111-Apoptosis,
pubmed-meshheading:16725111-Blotting, Western,
pubmed-meshheading:16725111-Calcium-Transporting ATPases,
pubmed-meshheading:16725111-Caspase 3,
pubmed-meshheading:16725111-Caspases,
pubmed-meshheading:16725111-Cell Adhesion,
pubmed-meshheading:16725111-Cell Line,
pubmed-meshheading:16725111-Cell Survival,
pubmed-meshheading:16725111-Cysteine Proteinase Inhibitors,
pubmed-meshheading:16725111-DNA-Binding Proteins,
pubmed-meshheading:16725111-Enzyme Activation,
pubmed-meshheading:16725111-Gene Expression,
pubmed-meshheading:16725111-Humans,
pubmed-meshheading:16725111-Isoenzymes,
pubmed-meshheading:16725111-Nuclear Proteins,
pubmed-meshheading:16725111-Oligopeptides,
pubmed-meshheading:16725111-RNA, Messenger,
pubmed-meshheading:16725111-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16725111-Sarcoplasmic Reticulum,
pubmed-meshheading:16725111-Sarcoplasmic Reticulum Calcium-Transporting ATPases,
pubmed-meshheading:16725111-Time Factors,
pubmed-meshheading:16725111-Transcription Factors,
pubmed-meshheading:16725111-Transfection
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pubmed:year |
2006
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pubmed:articleTitle |
Sarco/endoplasmic reticulum Ca2+ATPase type 3 isoforms (SERCA3b and SERCA3f): distinct roles in cell adhesion and ER stress.
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pubmed:affiliation |
Inserm U.689 E4, IFR-139, Centre de Recherche Cardiovasculaire Inserm Lariboisière, Hôpital Lariboisière, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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