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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2006-7-31
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pubmed:abstractText |
Avian influenza viruses (AIVs) are endemic in wild birds and their introduction and conversion to highly pathogenic avian influenza virus in domestic poultry is a cause of serious economic losses as well as a risk for potential transmission to humans. The ability to rapidly recognise AIVs in biological specimens is critical for limiting further spread of the disease in poultry. The advent of molecular methods such as real time polymerase chain reaction has allowed improvement of detection methods currently used in laboratories, although not all of these methods include an Internal Positive Control (IPC) to monitor for false negative results. Therefore we developed a one-step reverse transcription real time PCR (RRT-PCR) with a Minor Groove Binder (MGB) probe for the detection of different subtypes of AIVs. This technique also includes an IPC.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-10074505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-10606668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-10747142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-10799774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-11060074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-11684304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-12030760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-12202562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-12445938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-14599682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-15158597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-15283425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-15370041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-15709000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-1579108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-16102326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-16171460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-16252482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-16439323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-16461951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16725022-8610442
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-2334
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
87
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pubmed:dateRevised |
2010-9-16
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pubmed:meshHeading |
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pubmed:year |
2006
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pubmed:articleTitle |
A sensitive one-step real-time PCR for detection of avian influenza viruses using a MGB probe and an internal positive control.
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pubmed:affiliation |
Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità; V.le Regina Elena 299, 00161 Rome, Italy. ditrani@iss.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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