rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
30
|
pubmed:dateCreated |
2006-7-24
|
pubmed:abstractText |
Translation initiation factor 4E (eIF4E) is a cytoplasmic cap-binding protein that is required for cap-dependent translation initiation. Here, we have shown that eIF4E is ubiquitinated primarily at Lys-159 and incubation of cells with a proteasome inhibitor leads to increased eIF4E levels, suggesting the proteasome-dependent proteolysis of ubiquitinated eIF4E. Ubiquitinated eIF4E retained its cap binding ability, whereas eIF4E phosphorylation and eIF4G binding were reduced by ubiquitination. The W73A mutant of eIF4E exhibited enhanced ubiquitination/degradation, and 4E-BP overexpression protected eIF4E from ubiquitination/degradation. Because heat shock or the expression of the carboxyl terminus of heat shock cognate protein 70-interacting protein (Chip) dramatically increased eIF4E ubiquitination, Chip may be at least one ubiquitin E3 ligase responsible for eIF4E ubiquitination.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jul
|
pubmed:issn |
0021-9258
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
28
|
pubmed:volume |
281
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
20788-800
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:16720573-Cell Line,
pubmed-meshheading:16720573-Cytoplasm,
pubmed-meshheading:16720573-Eukaryotic Initiation Factor-4E,
pubmed-meshheading:16720573-Humans,
pubmed-meshheading:16720573-Lysine,
pubmed-meshheading:16720573-Mutation,
pubmed-meshheading:16720573-Phosphorylation,
pubmed-meshheading:16720573-Proteasome Endopeptidase Complex,
pubmed-meshheading:16720573-Protein Biosynthesis,
pubmed-meshheading:16720573-Protein Structure, Tertiary,
pubmed-meshheading:16720573-RNA Caps,
pubmed-meshheading:16720573-Sepharose,
pubmed-meshheading:16720573-Ubiquitin,
pubmed-meshheading:16720573-Ubiquitin-Protein Ligases
|
pubmed:year |
2006
|
pubmed:articleTitle |
Ubiquitination and proteasome-dependent degradation of human eukaryotic translation initiation factor 4E.
|
pubmed:affiliation |
Department of Viral Oncology, Institute for Virus Research, Kyoto University, Sakyo-ku, Japan.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|