Linked Life Data

16720320

Source:http://linkedlifedata.com/resource/pubmed/id/16720320

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SubjectPredicateObjectContext
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pubmed-article:16720320pubmed:dateCreated2006-5-24lld:pubmed
pubmed-article:16720320pubmed:abstractTextThe sperm membrane protein, designated as YWK-II protein/APLP2, is a member of the amyloid precursor protein (APP) superfamily and is a type I transmembrane protein involved in fertilization. Here, the structure-function of the domains of YWK-II protein was examined. Five segments with overlapping ends encompassing the entire extracellular region of mouse YWK-II gene were prepared, cloned and separately expressed in E. coli. The recombinant YWK-II segments were fused with glutathione S-transferase (GST), purified and evaluated for their antifertility activities by measuring their capacity to block in vitro mouse sperm-egg interaction. The structural domain(s) involved in the fertilization process was identified. The polypeptide segment corresponding to position 22-207 of YWK-II-763 inhibited the early stage of fertilization when the spermatozoa interacted with zona-free eggs; whereas the polypeptide segment 201-395 (lacking 309-364) of YWK-II-763 blocked sperm-egg membrane fusion. The remaining three segments, 201-395, 389-574 and 517-704 (lacking 613-624) of YWK-II-763, did not influence the in vitro fertilization process. The present results suggest that segment 22-308 of YWK-II-763 participates in the binding and fusion of sperm and egg plasma membranes thereby promoting fertilization.lld:pubmed
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pubmed-article:16720320pubmed:authorpubmed-author:WangYongYlld:pubmed
pubmed-article:16720320pubmed:authorpubmed-author:MiaoShiyingSlld:pubmed
pubmed-article:16720320pubmed:authorpubmed-author:WangLinfangLlld:pubmed
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pubmed-article:16720320pubmed:authorpubmed-author:QiaoYuanYlld:pubmed
pubmed-article:16720320pubmed:authorpubmed-author:ZhuangDazhong...lld:pubmed
pubmed-article:16720320pubmed:authorpubmed-author:YangChunboClld:pubmed
pubmed-article:16720320pubmed:issnTypeElectroniclld:pubmed
pubmed-article:16720320pubmed:volume11lld:pubmed
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pubmed-article:16720320pubmed:authorsCompleteYlld:pubmed
pubmed-article:16720320pubmed:pagination2371-80lld:pubmed
pubmed-article:16720320pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:16720320pubmed:year2006lld:pubmed
pubmed-article:16720320pubmed:articleTitleDelineation of the functional domains of the extracellular region of YWK-II Protein/APLP2 of sperm membrane.lld:pubmed
pubmed-article:16720320pubmed:affiliationNational Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Peking Union Medical College, 5 Dong Dan San, Tiao, Beijing, 100005, China.lld:pubmed
pubmed-article:16720320pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:16720320pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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