Source:http://linkedlifedata.com/resource/pubmed/id/16711254
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2006-5-22
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pubmed:abstractText |
Internal standard reference curves are used in enzyme-linked immunosorbent assay (ELISA) plates to control for inter- and intra-assay variance. To compare the diagnostic potential of multiple T. solium antigens on an unbiased, universal scale, we have created a standard curve using normal, human immunoglobulin G (hIgG). The hIgG curve is inexpensive and simple to prepare, and remains stable at 5 degrees C for at least one year, with a coefficient of variance of less than 10%. The hIgG standard curve has proven a critical tool for the comparison of several diagnostic antigens slated for assay development.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
1532-1819
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
173-81
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:16711254-Animals,
pubmed-meshheading:16711254-Antibodies, Helminth,
pubmed-meshheading:16711254-Antigens, Helminth,
pubmed-meshheading:16711254-Drug Stability,
pubmed-meshheading:16711254-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:16711254-Humans,
pubmed-meshheading:16711254-Immunoglobulin G,
pubmed-meshheading:16711254-Reference Standards,
pubmed-meshheading:16711254-Taenia solium,
pubmed-meshheading:16711254-Time Factors
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pubmed:year |
2006
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pubmed:articleTitle |
Development of a normal human immunoglobulin G standard curve for enzyme-linked immunosorbent assay: use for comparison of antigen efficacy.
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pubmed:affiliation |
Department of Cellular Biology, University of Georgia, Athens, USA. zsr3@cdc.gov
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pubmed:publicationType |
Journal Article,
Comparative Study
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