Linked Life Data

1671087

Source:http://linkedlifedata.com/resource/pubmed/id/1671087

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1991-2-26
pubmed:abstractText
The adrenergic receptor subtypes involved in cyclic AMP responses to norepinephrine (NE) were compared between slices of rat cerebral cortex and primary neuronal and glial cultures from rat brain. In neuronal cultures, NE and the beta-adrenergic receptor agonist isoproterenol (ISO) caused similar increases in cyclic AMP, which were not altered by the alpha-adrenergic receptor antagonist phentolamine. In glial cultures, NE caused a much smaller cyclic AMP response than did ISO, and this difference was reversed by alpha-adrenergic receptor antagonists (phentolamine greater than yohimbine greater than prazosin). alpha 2-Adrenergic receptor agonists partially inhibited the ISO response in glial cultures to a level similar to that observed with NE alone (clonidine = UK 14,304 greater than NE greater than 6-fluoro-NE greater than epinephrine). In slices from cerebral cortex, NE caused a much larger increase in cyclic AMP than did ISO, and this difference was reversed by alpha-adrenergic receptor antagonists with a different order of potency (prazosin greater than phentolamine greater than yohimbine). alpha 1-Adrenergic receptor agonists potentiated the response to ISO to a level similar to that observed with NE alone (epinephrine = NE greater than phenylephrine greater than 6-fluoro-NE greater than methoxamine). In all three tissue preparations, large responses to both alpha 1-receptor activation (increases in inositol phosphate accumulation) and alpha 2-receptor activation (decreases in forskolin-stimulated cyclic AMP accumulation) were observed. These data indicate that all of the major adrenergic receptor subtypes (beta, alpha 1, alpha 2) are present in each tissue preparation.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
587-95
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1671087-Adrenergic alpha-Agonists, pubmed-meshheading:1671087-Aging, pubmed-meshheading:1671087-Animals, pubmed-meshheading:1671087-Animals, Newborn, pubmed-meshheading:1671087-Cells, Cultured, pubmed-meshheading:1671087-Cerebral Cortex, pubmed-meshheading:1671087-Cyclic AMP, pubmed-meshheading:1671087-Drug Synergism, pubmed-meshheading:1671087-Forskolin, pubmed-meshheading:1671087-Isoproterenol, pubmed-meshheading:1671087-Kinetics, pubmed-meshheading:1671087-Neuroglia, pubmed-meshheading:1671087-Neurons, pubmed-meshheading:1671087-Norepinephrine, pubmed-meshheading:1671087-Phentolamine, pubmed-meshheading:1671087-Prazosin, pubmed-meshheading:1671087-Rats, pubmed-meshheading:1671087-Rats, Inbred Strains, pubmed-meshheading:1671087-Receptors, Adrenergic, alpha, pubmed-meshheading:1671087-Receptors, Adrenergic, beta, pubmed-meshheading:1671087-Yohimbine
pubmed:year
1991
pubmed:articleTitle
Multiple adrenergic receptor subtypes controlling cyclic AMP formation: comparison of brain slices and primary neuronal and glial cultures.
pubmed:affiliation
Department of Pharmacology, Emory University Medical School, Atlanta, Georgia 30322.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.