1670978

pubmed:Citation

1 Pt 1

Opioid receptors on isolated gastric smooth muscle cells were characterized pharmacologically by a technique in which synthetic selective opioid agonists and antagonists were used to protect and thus enrich a specific receptor type while all other receptors were inactivated by N-ethylamaleimide. Treatment of the cells with the selective mu-receptor agonist DAGO or antagonist CTAP preserved only the response to DAGO; treatment with the selective delta-receptor agonist DPDPE or antagonist naltrindole preserved only the response to DPPE; and treatment with the selective kappa-receptor agonist U50,488H or antagonist nor-binaltorphimine preserved only the response to U50,488H. The results established the presence of distinct kappa-, delta-, and mu-opioid receptors capable of mediating contraction of isolated gastric muscle cells. The pattern of interaction of endogenous opioid peptides with protected receptors implied that dynorphin-(1-13) and Met-enkephalin were selective agonists for kappa- and delta-opioid receptors, respectively, and Leu-enkephalin a preferential agonist of mu-opioid receptors. The results were confirmed by a reverse approach in which opioid receptors were inactivated by site-directed irreversible antagonists. beta-Funaltrexamine, a mu-selective antagonist, abolished the response to mu-receptor agonists, whereas beta-chlornaltrexamine, a mu- and kappa-selective antagonist, abolished the response to mu-receptor agonists and partially inhibited the response to kappa-receptor agonists.

eng

IM

MEDLINE

0002-9513

Print

NLM

G103-7

pubmed-meshheading:1670978-3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benz..., pubmed-meshheading:1670978-Analgesics, pubmed-meshheading:1670978-Animals, pubmed-meshheading:1670978-Dynorphins, pubmed-meshheading:1670978-Enkephalin, Ala(2)-MePhe(4)-Gly(5)-, pubmed-meshheading:1670978-Enkephalin, D-Penicillamine (2,5)-, pubmed-meshheading:1670978-Enkephalin, Leucine, pubmed-meshheading:1670978-Enkephalin, Methionine, pubmed-meshheading:1670978-Enkephalins, pubmed-meshheading:1670978-Guinea Pigs, pubmed-meshheading:1670978-Muscle, Smooth, pubmed-meshheading:1670978-Muscle Contraction, pubmed-meshheading:1670978-Peptide Fragments, pubmed-meshheading:1670978-Pyrrolidines, pubmed-meshheading:1670978-Receptors, Opioid, pubmed-meshheading:1670978-Receptors, Opioid, delta, pubmed-meshheading:1670978-Receptors, Opioid, kappa, pubmed-meshheading:1670978-Receptors, Opioid, mu, pubmed-meshheading:1670978-Sincalide, pubmed-meshheading:1670978-Stomach

Identification of opioid receptors on gastric muscle cells by selective receptor protection.

Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.