Linked Life Data

16705673

Source:http://linkedlifedata.com/resource/pubmed/id/16705673

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2006-5-23
pubmed:abstractText
alpha(1)-Adrenergic receptors (ARs) are not well defined in the central nervous system. The particular cell types and areas that express these receptors are uncertain because of the lack of high avidity antibodies and selective ligands. We have developed transgenic mice that either systemically overexpress the human alpha(1A)-AR subtype fused with the enhanced green fluorescent protein (EGFP) or express the EGFP protein alone under the control of the mouse alpha(1A)-AR promoter. We confirm our transgenic model against the alpha(1A)-AR knockout mouse, which expresses the LacZ gene in place of the coding region for the alpha(1A)-AR. By using these models, we have now determined cellular localization of the alpha(1A)-AR in the brain, at the protein level. The alpha(1A)-AR or the EGFP protein is expressed prominently in neuronal cells in the cerebral cortex, hippocampus, hypothalamus, midbrain, pontine olivary nuclei, trigeminal nuclei, cerebellum, and spinal cord. The types of neurons were diverse, and the alpha(1A)-AR colocalized with markers for glutamic acid decarboxylase (GAD), gamma-aminobutyric acid (GABA), and N-methyl-D-aspartate (NMDA) receptors. Recordings from alpha(1A)-AR EGFP-expressing cells in the stratum oriens of the hippocampal CA1 region confirmed that these cells were interneurons. We could not detect expression of the alpha(1A)-AR in mature astrocytes, oligodendrocytes, or cerebral blood vessels, but we could detect the alpha(1A)-AR in oligodendrocyte progenitors. We conclude that the alpha(1A)-AR is abundant in the brain, expressed in various types of neurons, and may regulate the function of oligodendrocyte progenitors, interneurons, GABA, and NMDA receptor containing neurons.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9967
pubmed:author
pubmed:copyrightInfo
Copyright 2006 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
497
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
209-22
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:16705673-Adrenergic alpha-1 Receptor Agonists, pubmed-meshheading:16705673-Animals, pubmed-meshheading:16705673-Antigens, pubmed-meshheading:16705673-Brain, pubmed-meshheading:16705673-Cell Differentiation, pubmed-meshheading:16705673-Gene Expression, pubmed-meshheading:16705673-Green Fluorescent Proteins, pubmed-meshheading:16705673-Immunohistochemistry, pubmed-meshheading:16705673-Membrane Potentials, pubmed-meshheading:16705673-Mice, pubmed-meshheading:16705673-Mice, Transgenic, pubmed-meshheading:16705673-Neurons, pubmed-meshheading:16705673-Norepinephrine, pubmed-meshheading:16705673-Oligodendroglia, pubmed-meshheading:16705673-Patch-Clamp Techniques, pubmed-meshheading:16705673-Proteoglycans, pubmed-meshheading:16705673-Radioligand Assay, pubmed-meshheading:16705673-Receptors, Adrenergic, alpha-1, pubmed-meshheading:16705673-Stem Cells, pubmed-meshheading:16705673-beta-Galactosidase, pubmed-meshheading:16705673-gamma-Aminobutyric Acid
pubmed:year
2006
pubmed:articleTitle
Localization of the mouse alpha1A-adrenergic receptor (AR) in the brain: alpha1AAR is expressed in neurons, GABAergic interneurons, and NG2 oligodendrocyte progenitors.
pubmed:affiliation
Department of Molecular Cardiology, Lerner Research Institute, The Cleveland Clinic Foundation, OH 44195, USA.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural