Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2006-9-7
pubmed:abstractText
Brief treatment with transforming growth factor (TGF)-beta1 stimulated the migration of macrophages, whereas long-term exposure decreased their migration. Cell migration stimulated by TGF-beta1 was markedly inhibited by 10 mug/mL Tat-C3 exoenzyme. TGF-beta1 increased mRNA and protein levels of macrophage inflammatory protein (MIP)-1alpha in the initial period, and these effects also were inhibited by 10 mug/mL Tat-C3 and a dominant-negative (DN)-RhoA (N19RhoA). Cycloheximide, actinomycin D, and antibodies against MIP-1alpha and monocyte chemoattractant protein-1 (MCP-1) abolished the stimulation of cell migration by TGF-beta1. These findings suggest that migration of these cells is regulated directly and indirectly via the expression of chemokines such as MIP-1alpha and MCP-1 mediated by RhoA in response to TGF-beta1. TGF-beta1 activated RhoA in the initial period, and thereafter inactivated them, suggesting that the inactivation of RhoA may be the cause of the reduced cell migration in response to TGF-beta1 at later times. We therefore attempted to elucidate the molecular mechanism of the inactivation of RhoA by TGF-beta1. First, TGF-beta1 phosphorylated RhoA via protein kinase A, leading to inactivation of RhoA. Second, wild-type p190 Rho GTPase activating protein (p190RhoGAP) reduced and DN-p190RhoGAP reversed the reduction of cell migration induced by TGF-beta, suggesting that it inactivated RhoA via p190 Rho GAP.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/ARHGAP35 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/ARHGAP5 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Arhgap5 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL3, http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL4, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/GTPase-Activating Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Grlf1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Guanine Nucleotide Exchange Factors, http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Inflammatory Proteins, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins, http://linkedlifedata.com/resource/pubmed/chemical/TGFB1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Tgfb1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta, http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta1, http://linkedlifedata.com/resource/pubmed/chemical/rho GTP-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/rhoA GTP-Binding Protein
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
108
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1821-9
pubmed:dateRevised
2011-9-27
pubmed:meshHeading
pubmed-meshheading:16705092-Animals, pubmed-meshheading:16705092-Base Sequence, pubmed-meshheading:16705092-Carrier Proteins, pubmed-meshheading:16705092-Cell Line, pubmed-meshheading:16705092-Cell Movement, pubmed-meshheading:16705092-Chemokine CCL3, pubmed-meshheading:16705092-Chemokine CCL4, pubmed-meshheading:16705092-Chemotaxis, pubmed-meshheading:16705092-Cyclic AMP-Dependent Protein Kinases, pubmed-meshheading:16705092-DNA-Binding Proteins, pubmed-meshheading:16705092-GTPase-Activating Proteins, pubmed-meshheading:16705092-Gene Expression, pubmed-meshheading:16705092-Guanine Nucleotide Exchange Factors, pubmed-meshheading:16705092-HL-60 Cells, pubmed-meshheading:16705092-Humans, pubmed-meshheading:16705092-Macrophage Activation, pubmed-meshheading:16705092-Macrophage Inflammatory Proteins, pubmed-meshheading:16705092-Macrophages, pubmed-meshheading:16705092-Mice, pubmed-meshheading:16705092-Models, Biological, pubmed-meshheading:16705092-RNA, Messenger, pubmed-meshheading:16705092-Repressor Proteins, pubmed-meshheading:16705092-Transforming Growth Factor beta, pubmed-meshheading:16705092-Transforming Growth Factor beta1, pubmed-meshheading:16705092-rho GTP-Binding Proteins, pubmed-meshheading:16705092-rhoA GTP-Binding Protein
pubmed:year
2006
pubmed:articleTitle
Transforming growth factor-beta1 regulates macrophage migration via RhoA.
pubmed:affiliation
Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Kangwon-Do 200-702, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't