Source:http://linkedlifedata.com/resource/pubmed/id/16701209
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2006-5-16
|
| pubmed:abstractText |
GABAB receptors are the G protein-coupled receptors for the main inhibitory neurotransmitter in the brain, gamma-aminobutyric acid (GABA). Molecular diversity in the GABAB system arises from the GABAB1a and GABAB1b subunit isoforms that solely differ in their ectodomains by a pair of sushi repeats that is unique to GABAB1a. Using a combined genetic, physiological, and morphological approach, we now demonstrate that GABAB1 isoforms localize to distinct synaptic sites and convey separate functions in vivo. At hippocampal CA3-to-CA1 synapses, GABAB1a assembles heteroreceptors inhibiting glutamate release, while predominantly GABAB1b mediates postsynaptic inhibition. Electron microscopy reveals a synaptic distribution of GABAB1 isoforms that agrees with the observed functional differences. Transfected CA3 neurons selectively express GABAB1a in distal axons, suggesting that the sushi repeats, a conserved protein interaction motif, specify heteroreceptor localization. The constitutive absence of GABAB1a but not GABAB1b results in impaired synaptic plasticity and hippocampus-dependent memory, emphasizing molecular differences in synaptic GABAB functions.
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| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0896-6273
|
| pubmed:author |
pubmed-author:BarbieriSamuelS,
pubmed-author:BettlerBernhardB,
pubmed-author:BiermannBarbaraB,
pubmed-author:Bräuner-OsborneHansH,
pubmed-author:CryanJohn FJF,
pubmed-author:FritschyJean-MarcJM,
pubmed-author:GassmannMartinM,
pubmed-author:GuetgNicoleN,
pubmed-author:JacobsonLaura HLH,
pubmed-author:KaupmannKlemensK,
pubmed-author:LujánRafaelR,
pubmed-author:MüllerMatthiasM,
pubmed-author:OertnerThomas GTG,
pubmed-author:SansigGillesG,
pubmed-author:ShigemotoRyuichiR,
pubmed-author:TurecekRostislavR,
pubmed-author:VacherClaire-MarieCM,
pubmed-author:VigotRéjanR,
pubmed-author:ZhangYan-PingYP
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| pubmed:issnType |
Print
|
| pubmed:day |
18
|
| pubmed:volume |
50
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
589-601
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:16701209-Animals,
pubmed-meshheading:16701209-Blotting, Northern,
pubmed-meshheading:16701209-Excitatory Postsynaptic Potentials,
pubmed-meshheading:16701209-Hippocampus,
pubmed-meshheading:16701209-Immunohistochemistry,
pubmed-meshheading:16701209-Memory,
pubmed-meshheading:16701209-Mice,
pubmed-meshheading:16701209-Mice, Mutant Strains,
pubmed-meshheading:16701209-Microscopy, Confocal,
pubmed-meshheading:16701209-Microscopy, Electron, Transmission,
pubmed-meshheading:16701209-Neuronal Plasticity,
pubmed-meshheading:16701209-Neurons,
pubmed-meshheading:16701209-Protein Isoforms,
pubmed-meshheading:16701209-Receptors, GABA-B,
pubmed-meshheading:16701209-Synapses,
pubmed-meshheading:16701209-Transfection
|
| pubmed:year |
2006
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| pubmed:articleTitle |
Differential compartmentalization and distinct functions of GABAB receptor variants.
|
| pubmed:affiliation |
Department of Clinical-Biological Sciences, Institute of Physiology, Pharmazentrum, University of Basel, CH-4056 Basel, Switzerland.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|