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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2006-5-15
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pubmed:abstractText |
We show that the antiapoptotic proteins BCL-2, BCL-XL, MCL-1, BFL-1, and BCL-w each bear a unique pattern of interaction with a panel of peptides derived from BH3 domains of BH3-only proteins. Cellular dependence on an antiapoptotic protein for survival can be decoded based on the pattern of mitochondrial sensitivity to this peptide panel, a strategy that we call BH3 profiling. Dependence on antiapoptotic proteins correlates with sequestration of activator BH3-only proteins like BID or BIM by antiapoptotic proteins. Sensitivity to the cell-permeable BCL-2 antagonist ABT-737 is also related to priming of BCL-2 by activator BH3-only molecules. Our data allow us to distinguish a cellular state we call "primed for death," which can be determined by BH3 profiling and which correlates with dependence on antiapoptotic family members for survival.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ABT-737,
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/BH3 Interacting Domain Death...,
http://linkedlifedata.com/resource/pubmed/chemical/Bax protein (53-86),
http://linkedlifedata.com/resource/pubmed/chemical/Bcl-2-like protein 11,
http://linkedlifedata.com/resource/pubmed/chemical/Biphenyl Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrophenols,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1535-6108
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
351-65
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:16697956-Amino Acid Sequence,
pubmed-meshheading:16697956-Animals,
pubmed-meshheading:16697956-Apoptosis,
pubmed-meshheading:16697956-Apoptosis Regulatory Proteins,
pubmed-meshheading:16697956-BH3 Interacting Domain Death Agonist Protein,
pubmed-meshheading:16697956-Biphenyl Compounds,
pubmed-meshheading:16697956-Cell Line, Tumor,
pubmed-meshheading:16697956-Enzyme Activators,
pubmed-meshheading:16697956-Intracellular Membranes,
pubmed-meshheading:16697956-Leukemia,
pubmed-meshheading:16697956-Membrane Proteins,
pubmed-meshheading:16697956-Mice,
pubmed-meshheading:16697956-Mitochondria, Liver,
pubmed-meshheading:16697956-Mitochondrial Membranes,
pubmed-meshheading:16697956-Molecular Sequence Data,
pubmed-meshheading:16697956-Nitrophenols,
pubmed-meshheading:16697956-Peptide Fragments,
pubmed-meshheading:16697956-Piperazines,
pubmed-meshheading:16697956-Protein Binding,
pubmed-meshheading:16697956-Proto-Oncogene Proteins,
pubmed-meshheading:16697956-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:16697956-Signal Transduction,
pubmed-meshheading:16697956-Sulfonamides
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pubmed:year |
2006
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pubmed:articleTitle |
Mitochondria primed by death signals determine cellular addiction to antiapoptotic BCL-2 family members.
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pubmed:affiliation |
Department of Medical Oncology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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