Linked Life Data

16695977

Source:http://linkedlifedata.com/resource/pubmed/id/16695977

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2010-6-28
pubmed:abstractText
DNA from archival Papanicolaou stained and unstained cytological smears was successfully isolated using a simple, rapid and inexpensive salting-out procedure. The quality of DNA was controlled by polymerase chain reaction (PCR) amplification of segments of the human beta-globin, human beta-actin and human papillomavirus L1 genes. Only negligible differences in amplification efficiency were observed between DNA isolated from stained and unstained smears. The salting-out procedure is a more rapid method for the isolation of DNA than phenol-chloroform extraction and may be used in instances where fresh or cryopreserved clinical specimens are not available.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Feb
pubmed:issn
1355-2910
pubmed:author
pubmed:issnType
Print
pubmed:volume
48
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
M55-6
pubmed:dateRevised
2010-9-14
pubmed:year
1995
pubmed:articleTitle
Isolation of DNA from archival Papanicolaou stained cytological smears using a simple salting-out procedure.
pubmed:affiliation
Institute of Microbiology, Medical Faculty of Ljubljana, Zaloska 4, 61105 Ljubljana, Slovenia.
pubmed:publicationType
Journal Article