Linked Life Data

16684779

Source:http://linkedlifedata.com/resource/pubmed/id/16684779

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
28
pubmed:dateCreated
2006-7-10
pubmed:abstractText
Recently dynein light chain 1 (DLC1), a cytoskeleton signaling component, has been shown to interact with and transactivate estrogen receptor-alpha (ER), leading to increased expression of ER target genes and growth stimulation of breast cancer cells. However, the molecular mechanism by which DLC1 regulates the ER pathway remains poorly understood. To gain insights into the putative mechanism, here we set out to identify novel DLC1-interacting proteins. We identified KIBRA, a WW domain- and a glutamic acid stretch-containing protein, as a DLC1-binding protein and showed that it interacts with DLC1 both in vitro and in vivo. We found that KIBRA-DLC1 complex is recruited to ER-responsive promoters. We also found that KIBRA-DLC1 interaction is mandatory for the recruitment and transactivation functions of ER or DLC1 to the target chromatin. Finally we found that KIBRA interacts with histone H3 via its glutamic acid-rich region and that such interaction might play a mechanistic role in conferring an optimal ER transactivation function as well as the proliferation of ligand-stimulated breast cancer cells. Together these findings indicate that DLC1-KIBRA interaction is essential for ER transactivation in breast cancer cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
281
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19092-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:16684779-Cell Line, Tumor, pubmed-meshheading:16684779-Chromatin, pubmed-meshheading:16684779-Cloning, Molecular, pubmed-meshheading:16684779-Cytoplasmic Dyneins, pubmed-meshheading:16684779-Cytoskeleton, pubmed-meshheading:16684779-Dyneins, pubmed-meshheading:16684779-Estrogen Receptor alpha, pubmed-meshheading:16684779-Glutamic Acid, pubmed-meshheading:16684779-Histones, pubmed-meshheading:16684779-Humans, pubmed-meshheading:16684779-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:16684779-Phosphoproteins, pubmed-meshheading:16684779-Promoter Regions, Genetic, pubmed-meshheading:16684779-Protein Binding, pubmed-meshheading:16684779-Protein Structure, Tertiary, pubmed-meshheading:16684779-Proteins, pubmed-meshheading:16684779-Transcriptional Activation
pubmed:year
2006
pubmed:articleTitle
Essential role of KIBRA in co-activator function of dynein light chain 1 in mammalian cells.
pubmed:affiliation
Department of Molecular and Cellular Oncology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural