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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2006-6-19
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pubmed:abstractText |
In this study we have developed and validated a novel approach of transgene regulation in the brain. By using lentiviral vectors that incorporate promoters of genes that are up-regulated during different pathological states, we were able to regulate transgene expression in accordance with the disease process. When using a glial fibrillary acidic protein promoter, efficient disease regulation in glial cells was achieved after an excitotoxic lesion or a 6-hydroxydopamine (6-OHDA) lesion. Transgene expression was physiologically regulated and displayed a dose-dependent increase depending on the severity of lesion. Efficient regulation was also achieved in neurons when using a preproenkephlin promoter in 6-OHDA-lesioned rats, allowing combined regulation and targeting. This disease-regulated approach allows control of transgene expression in the brain without the use of inducer molecules and without overexpression of transactivator proteins.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0360-4012
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2006 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
58-67
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:16683227-Animals,
pubmed-meshheading:16683227-Animals, Genetically Modified,
pubmed-meshheading:16683227-Brain,
pubmed-meshheading:16683227-Brain Injuries,
pubmed-meshheading:16683227-Functional Laterality,
pubmed-meshheading:16683227-Gene Expression Regulation,
pubmed-meshheading:16683227-Genetic Vectors,
pubmed-meshheading:16683227-Glial Fibrillary Acidic Protein,
pubmed-meshheading:16683227-Green Fluorescent Proteins,
pubmed-meshheading:16683227-Humans,
pubmed-meshheading:16683227-Ibotenic Acid,
pubmed-meshheading:16683227-Immunohistochemistry,
pubmed-meshheading:16683227-Lentivirus,
pubmed-meshheading:16683227-Nerve Tissue Proteins,
pubmed-meshheading:16683227-Oxidopamine,
pubmed-meshheading:16683227-Promoter Regions, Genetic,
pubmed-meshheading:16683227-RNA, Messenger,
pubmed-meshheading:16683227-Rats,
pubmed-meshheading:16683227-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:16683227-Time Factors,
pubmed-meshheading:16683227-beta-Galactosidase
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pubmed:year |
2006
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pubmed:articleTitle |
Evidence for disease-regulated transgene expression in the brain with use of lentiviral vectors.
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pubmed:affiliation |
Department of Experimental Medical Research, Section for Neuroscience, Wallenberg Neuroscience Centre, Lund University, Lund, Sweden.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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