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rdf:type |
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lifeskim:mentions |
umls-concept:C0010423,
umls-concept:C0017262,
umls-concept:C0043309,
umls-concept:C0061132,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0439611,
umls-concept:C1333714,
umls-concept:C1514562,
umls-concept:C1880022,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C1998793,
umls-concept:C2911684
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pubmed:issue |
Pt 5
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pubmed:dateCreated |
2006-5-9
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pubmed:abstractText |
Galectins are a family of animal lectins which share similar carbohydrate-recognition domains (CRDs) and an affinity for beta-galactosides. A novel human galectin-related protein named GRP (galectin-related protein; previously known as HSPC159) comprises only one conserved CRD with 38 additional N-terminal residues. The C-terminal fragment of human GRP (GRP-C; residues 38-172) containing the CRD has been expressed and purified. The protein was crystallized using the hanging-drop vapour-diffusion method from a solution containing 2% PEG 400 and 2M ammonium sulfate in 100 mM Tris-HCl buffer pH 7.5. Diffraction data were collected to a resolution limit of 2.0 angstroms at beamline 3W1A of Beijing Synchrotron Radiation Facility at 100 K. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 123.07, b = 96.67, c = 61.56 angstroms, beta = 118.72 degrees. The estimated Matthews coefficient was 2.6 angstroms3 Da(-1), corresponding to 51.8% solvent content.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-10467344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-10702423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-11027447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-11435439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-12223271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-15148380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-1639789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-5339877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-5700707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-7501023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-7862663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-7890611,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-8050350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-8063692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-8274188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-8280461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-8347574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-9151710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-9153289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-9234729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682780-9625297
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1744-3091
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
474-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2006
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pubmed:articleTitle |
Expression, purification, crystallization and preliminary X-ray characterization of the GRP carbohydrate-recognition domain from Homo sapiens.
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pubmed:affiliation |
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230027, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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