Linked Life Data

16682768

Source:http://linkedlifedata.com/resource/pubmed/id/16682768

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 5
pubmed:dateCreated
2006-5-9
pubmed:abstractText
Phosphoribosyl pyrophosphate synthetase (PRS; EC 2.7.6.1) catalyzes the reaction of ribose-5-phosphate (R5P) with ATP to yield AMP and PRPP (5-phosphoribosyl-1-pyrophosphate), which is necessary for the de novo and salvage pathways of purine-, pyrimidine- and pyridine-nucleotide biosynthesis. PRPP is a metabolite that is required at all times in the cell and is thus central to life. In this study, human PRS1 was produced in Escherichia coli in soluble form and purified to homogeneity. Crystals in complex with Mg2+, inorganic phosphate (P(i)) and ATP were obtained by the hanging-drop vapour-diffusion method. Diffraction data were collected to 2.6 A resolution. The crystal belongs to space group R3, with unit-cell parameters a = b = 168.846, c = 61.857 angstroms, assuming two molecules in the asymmetric unit and a volume-to-weight ratio of 2.4 angstroms3 Da(-1), which was consistent with the result calculated from the self-rotation function.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/16682768-170262, http://linkedlifedata.com/resource/pubmed/commentcorrection/16682768-171280, http://linkedlifedata.com/resource/pubmed/commentcorrection/16682768-2156819, http://linkedlifedata.com/resource/pubmed/commentcorrection/16682768-2537655, http://linkedlifedata.com/resource/pubmed/commentcorrection/16682768-2542328, http://linkedlifedata.com/resource/pubmed/commentcorrection/16682768-3009477, http://linkedlifedata.com/resource/pubmed/commentcorrection/16682768-4306285, http://linkedlifedata.com/resource/pubmed/commentcorrection/16682768-4346548, http://linkedlifedata.com/resource/pubmed/commentcorrection/16682768-6123809, http://linkedlifedata.com/resource/pubmed/commentcorrection/16682768-6243137, http://linkedlifedata.com/resource/pubmed/commentcorrection/16682768-7593598, http://linkedlifedata.com/resource/pubmed/commentcorrection/16682768-8702702
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1744-3091
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
432-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2006
pubmed:articleTitle
Expression, purification, crystallization and preliminary X-ray diffraction analysis of human phosphoribosyl pyrophosphate synthetase 1 (PRS1).
pubmed:affiliation
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230027, People's Republic of China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't