Linked Life Data

16680144

Source:http://linkedlifedata.com/resource/pubmed/id/16680144

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2006-5-22
pubmed:abstractText
Immunoglobulin rearrangement from variable heavy chain (V(H)) to diversity (D)-joining heavy chain (J(H)), which occurs exclusively in B lineage cells, is impaired in mice deficient for the B lineage-specific transcription factor Pax5. Conversely, ectopic Pax5 expression in thymocytes promotes the rearrangement of D(H)-proximal V(H)7183 genes. In exploring the mechanism for Pax5 regulation of V(H)-to-DJ(H) recombination, we have identified multiple Pax5 binding sites in the coding regions of human and mouse V(H) gene segments. Pax5 bound to those sites in vitro and occupied V(H) genes in early human and mouse B lineage cells. Moreover, Pax5 interacted with the recombination-activating gene 1 (RAG1)-RAG2 complex to enhance RAG-mediated V(H) recombination signal sequence cleavage and recombination of a V(H) gene substrate. These findings indicate a direct activating function for Pax5 in RAG-mediated immunoglobulin V(H)-to-DJ(H) recombination.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1529-2908
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
616-24
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:16680144-Animals, pubmed-meshheading:16680144-B-Cell-Specific Activator Protein, pubmed-meshheading:16680144-Binding Sites, pubmed-meshheading:16680144-Cell Lineage, pubmed-meshheading:16680144-DNA-Binding Proteins, pubmed-meshheading:16680144-Gene Rearrangement, B-Lymphocyte, Heavy Chain, pubmed-meshheading:16680144-Genes, Immunoglobulin, pubmed-meshheading:16680144-Homeodomain Proteins, pubmed-meshheading:16680144-Humans, pubmed-meshheading:16680144-Immunoglobulin Joining Region, pubmed-meshheading:16680144-Immunoglobulin Variable Region, pubmed-meshheading:16680144-Mice, pubmed-meshheading:16680144-Mice, Mutant Strains, pubmed-meshheading:16680144-Nuclear Proteins, pubmed-meshheading:16680144-Protein Interaction Mapping, pubmed-meshheading:16680144-Transcriptional Activation
pubmed:year
2006
pubmed:articleTitle
Transcription factor Pax5 (BSAP) transactivates the RAG-mediated V(H)-to-DJ(H) rearrangement of immunoglobulin genes.
pubmed:affiliation
Division of Developmental and Clinical Immunology, University of Alabama at Birmingham, 35294, USA. zhixin.zhang@ccc.uab.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural