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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2006-6-6
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pubmed:abstractText |
In traditional screening with 96-well plates, microliters of substrates are consumed for each reaction. Further miniaturization is limited by the special equipment and techniques required to dispense nanoliter volumes of fluid. Plug-based microfluidics confines reagents in nanoliter plugs (droplets surrounded by fluorinated carrier fluid), and uses simple pumps to control the flow of plugs. By using cartridges pre-loaded with nanoliter plugs of reagents, only two pumps and a merging junction are needed to set up a screen. Screening with preloaded cartridges uses only nanoliters of substrate per reaction, and requires no microfabrication. The low cost and simplicity of this method has the potential of replacing 96-well and other multi-well plates, and has been applied to enzymatic assays, protein crystallization and optimization of organic reactions.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-11002279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-14624612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-15269797,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-15373431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-15452343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-15465312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-15565706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-15623279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-15638474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-15679345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-15871692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-15931170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-16154351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-16220918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-16357255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-16448074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-16472133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-16492019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/16677848-16543450
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1367-5931
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
226-31
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pubmed:dateRevised |
2010-12-3
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pubmed:meshHeading |
pubmed-meshheading:16677848-Chemical Precipitation,
pubmed-meshheading:16677848-Crystallization,
pubmed-meshheading:16677848-Drug Evaluation, Preclinical,
pubmed-meshheading:16677848-Indicators and Reagents,
pubmed-meshheading:16677848-Microfluidics,
pubmed-meshheading:16677848-Nanotechnology,
pubmed-meshheading:16677848-Proteins,
pubmed-meshheading:16677848-Spectrometry, Fluorescence,
pubmed-meshheading:16677848-Spectrometry, Mass, Matrix-Assisted Laser...
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pubmed:year |
2006
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pubmed:articleTitle |
Microfluidic cartridges preloaded with nanoliter plugs of reagents: an alternative to 96-well plates for screening.
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pubmed:affiliation |
Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 5735 South Ellis Avenue, Chicago, Illinois 60637, USA.
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pubmed:publicationType |
Journal Article,
Review,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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