Linked Life Data

16675598

Source:http://linkedlifedata.com/resource/pubmed/id/16675598

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2006-7-26
pubmed:databankReference
pubmed:abstractText
Human embryonic stem cells (HESCs) are pluripotent cells that may serve as a source of cells for transplantation medicine and as a tool to study human embryogenesis. Using genetic manipulation methodologies, we have investigated the potential of HESCs to differentiate into the various pancreatic cell types. We initially created various HESCs carrying the enhanced green fluorescent protein (eGFP) reporter gene under the control of either the insulin promoter or the pancreatic and duodenal homeobox factor-1 (Pdx1) promoter. Our analysis revealed that during the differentiation of HESCs into embryoid bodies (EBs), we could detect green fluorescent cells when eGFP is regulated by Pdx1 promoter but not by insulin promoter. To examine whether we can induce differentiation into pancreatic cells, we have established human embryonic stem cell lines that constitutively express either Pdx1 or the endodermal transcription factor Foxa2. Following differentiation into EBs, the constitutive expression of Pdx1 enhanced the differentiation of HESCs toward pancreatic endocrine and exocrine cell types. Thus, we have demonstrated expression of several transcription factors that are downstream of Pdx1 and various molecular markers for the different pancreatic cell types. However, the expression of the insulin gene could be demonstrated only when the cells differentiated in vivo into teratomas. We conclude that although overexpression of Pdx1 enhanced expression of pancreatic enriched genes, induction of insulin expression may require additional signals that are only present in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1066-5099
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1923-30
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:16675598-Animals, pubmed-meshheading:16675598-Cell Differentiation, pubmed-meshheading:16675598-Cell Line, pubmed-meshheading:16675598-Disease Models, Animal, pubmed-meshheading:16675598-Embryonic Stem Cells, pubmed-meshheading:16675598-Gene Expression Profiling, pubmed-meshheading:16675598-Gene Expression Regulation, Developmental, pubmed-meshheading:16675598-Green Fluorescent Proteins, pubmed-meshheading:16675598-Hepatocyte Nuclear Factor 3-beta, pubmed-meshheading:16675598-Homeodomain Proteins, pubmed-meshheading:16675598-Humans, pubmed-meshheading:16675598-Insulin, pubmed-meshheading:16675598-Mice, pubmed-meshheading:16675598-Mice, SCID, pubmed-meshheading:16675598-Molecular Sequence Data, pubmed-meshheading:16675598-Pancreas, pubmed-meshheading:16675598-Promoter Regions, Genetic, pubmed-meshheading:16675598-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:16675598-Teratoma, pubmed-meshheading:16675598-Trans-Activators, pubmed-meshheading:16675598-Transplantation, Heterologous
pubmed:year
2006
pubmed:articleTitle
The effect of overexpression of Pdx1 and Foxa2 on the differentiation of human embryonic stem cells into pancreatic cells.
pubmed:affiliation
Department of Genetics, The Hebrew University, Jerusalem 91904, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't